liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis
Texas A&M University.
University of Gothenburg.
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
University of Gothenburg.
Show others and affiliations
2011 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 157, no 4, 1765-1777 p.Article in journal (Refereed) Published
Abstract [en]

Nonphotosynthetic plastids are important sites for the biosynthesis of starch, fatty acids, and amino acids. The uptake and subsequent use of cytosolic ATP to fuel these and other anabolic processes would lead to the accumulation of inorganic phosphate (Pi) if not balanced by a Pi export activity. However, the identity of the transporter(s) responsible for Pi export is unclear. The plastid-localized Pi transporter PHT4;2 of Arabidopsis (Arabidopsis thaliana) is expressed in multiple sink organs but is nearly restricted to roots during vegetative growth. We identified and used pht4;2 null mutants to confirm that PHT4; 2 contributes to Pi transport in isolated root plastids. Starch accumulation was limited in pht4; 2 roots, which is consistent with the inhibition of starch synthesis by excess Pi as a result of a defect in Pi export. Reduced starch accumulation in leaves and altered expression patterns for starch synthesis genes and other plastid transporter genes suggest metabolic adaptation to the defect in roots. Moreover, pht4; 2 rosettes, but not roots, were significantly larger than those of the wild type, with 40% greater leaf area and twice the biomass when plants were grown with a short (8-h) photoperiod. Increased cell proliferation accounted for the larger leaf size and biomass, as no changes were detected in mature cell size, specific leaf area, or relative photosynthetic electron transport activity. These data suggest novel signaling between roots and leaves that contributes to the regulation of leaf size.

Place, publisher, year, edition, pages
American Society of Plant Biologists , 2011. Vol. 157, no 4, 1765-1777 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-74862DOI: 10.1104/pp.111.181925ISI: 000298375600014OAI: diva2:496533
Funding Agencies|National Science Foundation|IOS-0416443IOS-0956486|Swedish Research Council|622-2007-517621-2007-5440|Swedish Research Council for Environment, Agriculture, and Space Planning|229-2007-1378|Available from: 2012-02-10 Created: 2012-02-10 Last updated: 2012-02-15

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Karlsson, PatrikKuruvilla, JacobSpetea Wiklund, Cornelia
By organisation
Department of Clinical and Experimental MedicineFaculty of Health Sciences
In the same journal
Plant Physiology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 75 hits
ReferencesLink to record
Permanent link

Direct link