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Selective determination of urea using urease immobilized on ZnO nanowires
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Acromed Invest AB, SE-22643 Lund, Sweden .
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
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2011 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 160, no 1, 637-643 p.Article in journal (Refereed) Published
Abstract [en]

Well-aligned zinc oxide (ZnO) nanowire arrays were fabricated on gold-coated plastic substrates using a low-temperature aqueous chemical growth (ACG) method. The ZnO nanowire arrays with 50-130 nm diameters and similar to 1 mu m in lengths were used in an enzyme-based urea sensor through immobilization of the enzyme urease that was found to be sensitive to urea concentrations from 0.1 mM to 100 mM. Two linear sensitivity regions were observed when the electrochemical responses (EMF) of the sensors were plotted vs. the logarithmic concentration range of urea from 0.1 mM to 100 mM. The proposed sensor showed a sensitivity of 52.8 mV/decade for 0.1-40 mM urea and a fast response time less than 4s was achieved with good selectivity, reproducibility and negligible response to common interferents such as ascorbic acid and uric acid, glucose. K(+) and Na(+) ions.

Place, publisher, year, edition, pages
Elsevier , 2011. Vol. 160, no 1, 637-643 p.
Keyword [en]
ZnO-nanowire arrays, Electrochemical nanodevices, Urease enzyme, Urea determination
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-74860DOI: 10.1016/j.snb.2011.08.041ISI: 000298768100090OAI: oai:DiVA.org:liu-74860DiVA: diva2:496537
Available from: 2012-02-10 Created: 2012-02-10 Last updated: 2017-12-07

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Usman Ali, SyedIbupoto, Zafar HussainNur, OmerWillander, Magnus

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