Selective determination of urea using urease immobilized on ZnO nanowires
2011 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, Vol. 160, no 1, 637-643 p.Article in journal (Refereed) Published
Well-aligned zinc oxide (ZnO) nanowire arrays were fabricated on gold-coated plastic substrates using a low-temperature aqueous chemical growth (ACG) method. The ZnO nanowire arrays with 50-130 nm diameters and similar to 1 mu m in lengths were used in an enzyme-based urea sensor through immobilization of the enzyme urease that was found to be sensitive to urea concentrations from 0.1 mM to 100 mM. Two linear sensitivity regions were observed when the electrochemical responses (EMF) of the sensors were plotted vs. the logarithmic concentration range of urea from 0.1 mM to 100 mM. The proposed sensor showed a sensitivity of 52.8 mV/decade for 0.1-40 mM urea and a fast response time less than 4s was achieved with good selectivity, reproducibility and negligible response to common interferents such as ascorbic acid and uric acid, glucose. K(+) and Na(+) ions.
Place, publisher, year, edition, pages
Elsevier , 2011. Vol. 160, no 1, 637-643 p.
ZnO-nanowire arrays, Electrochemical nanodevices, Urease enzyme, Urea determination
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-74860DOI: 10.1016/j.snb.2011.08.041ISI: 000298768100090OAI: oai:DiVA.org:liu-74860DiVA: diva2:496537