Bioreactor optimization of tissue engineered rabbit flexor tendons in vivo
2012 (English)In: JOURNAL OF HAND SURGERY-EUROPEAN VOLUME, ISSN 1753-1934, Vol. 37E, no 2, 109-114 p.Article in journal (Refereed) Published
Tissue-engineered rabbit flexor tendons reseeded with cells are stronger in vitro after culture in a bioreactor. It is not known whether this effect persists in vivo. Tenocytes from New Zealand white rabbits were seeded onto rabbit rear paw flexor tendons that were deprived of cells and exposed to cyclic strain in a bioreactor. Reseeded constructs that were kept unloaded in a medium for 5 days were used as controls. The tendons were implanted to bridge a zone II defect in the rabbit. After explantation 4 weeks later, the ultimate tensile strength (UTS) and elastic modulus (EM) were determined. Tendon constructs that were exposed to cyclic strain had significantly improved UTS and EM. Histology showed that cellularity was increased in the bioreactor tendons.
Place, publisher, year, edition, pages
SAGE Publications (UK and US) , 2012. Vol. 37E, no 2, 109-114 p.
Hand flexor tendon, tissue engineering, tenocytes, bioreactor optimization, cell culture
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-75278DOI: 10.1177/1753193411419439ISI: 000299481700003OAI: oai:DiVA.org:liu-75278DiVA: diva2:505942
Funding Agencies|Veterans Affairs Medical Merit Review Grant||Veterans Affairs Rehabilitation Research & Development Merit Review Grant||Swedish Fulbright Commission||County Council of Ostergotland (Sweden)||Borje Gabrielssons Memorial Fund||Swedish Society of Medicine||Johan & Jakob Soderberg Foundation||Linkoping Society of Medicine||2012-02-272012-02-242012-02-27