Epithelial Expression of Vasoactive Intestinal Peptide in Ulcerative Colitis: Down-Regulation in Markedly Inflamed Colon
2012 (English)In: Digestive Diseases and Sciences, ISSN 0163-2116, E-ISSN 1573-2568, Vol. 57, no 2, 303-310 p.Article in journal (Refereed) Published
Vasoactive intestinal peptide (VIP) has a number of important effects in intestinal physiology and pathology, including in ulcerative colitis (UC). The expression patterns of the predominant VIP receptor in the mucosa (the VPAC1 receptor) are unknown for the mucosa in UC. It is assumed that the sources of VIP in the intestine are the innervation and the inflammatory cells. less thanbrgreater than less thanbrgreater thanThe VIP and VPAC1 receptor expression patterns in the epithelial layer of UC and non-UC patients were examined in the present study. The influence of marked inflammation of the mucosa was evaluated. less thanbrgreater than less thanbrgreater thanSpecimens of the human colon, including the colon of UC patients, were examined concerning expressions of VIP and VPAC1 receptor, focusing on the epithelial layer. Immunohistochemistry and in situ hybridization were utilized. less thanbrgreater than less thanbrgreater thanThere were VIP mRNA reactions and also marked VPAC1 receptor immunoreactions in the normal and slightly/moderately affected epithelium. VIP mRNA reactions were not detected and VPAC1 immunoreactions were minimal in response to marked mucosal derangement. less thanbrgreater than less thanbrgreater thanThe findings suggest that there is a local production of VIP in the epithelial cells in normal and slightly/moderately inflamed mucosa but not in severely inflamed mucosa. Furthermore, a marked downregulation in VPAC1 receptor expressions occurs in the epithelium in severe UC. Based on the knowledge that VIP can have trophic, healing and anti-inflammatory effects, it is likely that the decrease in VIP mRNA and VPAC1 receptor reactions seen in severely affected mucosa in UC may be associated with adverse effects on intestinal function.
Place, publisher, year, edition, pages
Springer Verlag (Germany) , 2012. Vol. 57, no 2, 303-310 p.
Colitis, Colonic epithelium, Neuropeptide, VIP, VPAC1
IdentifiersURN: urn:nbn:se:liu:diva-75274DOI: 10.1007/s10620-011-1985-3ISI: 000299487500008OAI: oai:DiVA.org:liu-75274DiVA: diva2:505948
Funding Agencies|Lions Cancer Foundation||Faculty of Medicine, Umea University||2012-02-272012-02-242012-02-27