Single Layer Centrifugation of Stallion Spermatozoa through Androcoll (TM)-E does not Adversely Affect their Capacitation-Like Status, as Measured by CTC Staining
2011 (English)In: Reproduction in domestic animals (1990), ISSN 0936-6768, E-ISSN 1439-0531, Vol. 46, no 1, e74-e78 p.Article in journal (Refereed) Published
Contents This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation-like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6 degrees C. Sperm capacitation-like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation-like status between colloid-selected and non-selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome-reacted spermatozoa. In conclusion, SLC through Androcoll (TM)-E does not adversely affect the capacitation-like status of stallion spermatozoa, although it did increase with time during cold storage.
Place, publisher, year, edition, pages
Blackwell Publishing, 2011. Vol. 46, no 1, e74-e78 p.
IdentifiersURN: urn:nbn:se:liu:diva-75537DOI: 10.1111/j.1439-0531.2010.01637.xISI: 000286382700021OAI: oai:DiVA.org:liu-75537DiVA: diva2:508064