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GroEL-induced stretching of a substrate protein: An EPR/SDSL study in BIOPHYSICAL JOURNAL, vol 88, issue 1, pp 562A-562A
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Molecular genetics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biochemistry. Linköping University, The Institute of Technology.
University of Denver, Department of Chemistry & Biochemistry Denver, USA.
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2005 (English)In: BIOPHYSICAL JOURNAL, Elsevier (Cell Press) / Biophysical Society , 2005, Vol. 88, no 1, 562A-562A p.Conference paper, Published paper (Refereed)
Abstract [en]

The Hsp60-type chaperonin GroEL assists in the folding of the enzyme Human Carbonic Anhydrase II (HCA II) and protects it from aggregation.It is still a controversy whether the action of GroEL is an active or passive process. Single- and double-cysteine mutants were specificallyspin labeled at a topological breakpoint in the β-core of HCA II. X-band electron paramagnetic resonance (EPR) was used at physiologicaltemperatures to monitor the GroEL-induced structural changes in this region of HCA II. Inter-residue distance calculations based on dipolarinteraction show that the proximity of the labeled positions F147 and K213 in the native state of HCA II is ~11±2 Å, and that it is virtuallyintact in the thermally-induced molten-globule state that binds to GroEL. However, upon interaction with GroEL a spin-spin distance increaseto ~22±3 Å indicates a conformational change in HCA II that is part of the GroEL-induced substrate stretch that enables structural rearrangementof a misfolded substrate protein.

Place, publisher, year, edition, pages
Elsevier (Cell Press) / Biophysical Society , 2005. Vol. 88, no 1, 562A-562A p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-75802ISI: 000226378502751OAI: oai:DiVA.org:liu-75802DiVA: diva2:509103
Conference
48th Rocky Mountain Conference on Analytical Chemistry, July 23-26, Breckenridge, USA
Available from: 2012-03-12 Created: 2012-03-12 Last updated: 2013-11-19Bibliographically approved

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Owenius, RikardJarl, AnngelicaCarlsson, UnoHammarström, Per

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