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Low diversity of the gut microbiota in infants with atopic eczema
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping.
Department of Preparedness, Swedish Institute for Communicable Disease Control, Solna, Sweden.
Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden.
Institute of Environmental Medicine, Karolinska Institutet, Stockholm, and the School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
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2012 (English)In: Journal of Allergy and Clinical Immunology, ISSN 0091-6749, E-ISSN 1097-6825, Vol. 129, no 2, 434-440 p.Article in journal (Refereed) Published
Abstract [en]

Background

It is debated whether a low total diversity of the gut microbiota in early childhood is more important than an altered prevalence of particular bacterial species for the increasing incidence of allergic disease. The advent of powerful, cultivation-free molecular methods makes it possible to characterize the total microbiome down to the genus level in large cohorts.

Objective

We sought to assess microbial diversity and characterize the dominant bacteria in stool during the first year of life in relation to atopic eczema development.

Methods

Microbial diversity and composition were analyzed with barcoded 16S rDNA 454-pyrosequencing in stool samples at 1 week, 1 month, and 12 months of age in 20 infants with IgE-associated eczema and 20 infants without any allergic manifestation until 2 years of age (ClinicalTrials.gov ID NCT01285830).

Results

Infants with IgE-associated eczema had a lower diversity of the total microbiota at 1 month (P = .004) and a lower diversity of the bacterial phylum Bacteroidetes and the genus Bacteroides at 1 month (P = .02 and P = .01) and the phylum Proteobacteria at 12 months of age (P = .02). The microbiota was less uniform at 1 month than at 12 months of age, with a high interindividual variability. At 12 months, when the microbiota had stabilized, Proteobacteria, comprising gram-negative organisms, were more abundant in infants without allergic manifestation (Empirical Analysis of Digital Gene Expression in R [edgeR] test: P = .008, q = 0.02).

Conclusion

Low intestinal microbial diversity during the first month of life was associated with subsequent atopic eczema.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 129, no 2, 434-440 p.
Keyword [en]
Allergic disease, Bacteroides species, diversity, eczema, hygiene hypothesis, infant, microbiota, molecular microbiology, pyrosequencing, Sutterella species
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-75901DOI: 10.1016/j.jaci.2011.10.025ISI: 000299951700021PubMedID: 22153774OAI: oai:DiVA.org:liu-75901DiVA: diva2:510548
Note

Funding Agencies|BioGaia AB, Stockholm, Sweden||Ekhaga Foundation, the Heart and Lung foundation||Research Council for the South-East Sweden|F2000-106|Olle Engqvist Foundation||Swedish Asthma and Allergy Association||Swedish Research Council||University Hospital of Linkoping||Soderberg Foundation||Vardal Foundation for Health Care Science and Allergy Research, Sweden||BioGaia AB||

Available from: 2012-03-16 Created: 2012-03-16 Last updated: 2017-12-07

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Abrahamsson, ThomasJenmalm, Maria

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