Cold-induced activation of brown adipose tissue and adipose angiogenesis in mice
2012 (English)In: Nature Protocols, ISSN 1754-2189, Vol. 7, no 3, 606-615 p.Article in journal (Refereed) Published
Exposure of humans and rodents to cold activates thermogenic activity in brown adipose tissue (BAT). This protocol describes a mouse model to study the activation of BAT and angiogenesis in adipose tissues by cold acclimation. After a 1-week exposure to 4 degrees C, adult C57BL/6 mice show an obvious transition from subcutaneous white adipose tissue (WAT) into brown-like adipose tissue (BRITE). The BRITE phenotype persists after continuous cold exposure, and by the end of week 5 BRITE contains a high number of uncoupling protein-1-positive mitochondria, a characteristic feature of BAT. During the transition from WAT into BRITE, the vascular density is markedly increased owing to the activation of angiogenesis. In BAT, cold exposure stimulates thermogenesis by increasing the mitochondrial content and metabolic rate. BAT and the increased metabolic rate result in a lean phenotype. This protocol provides an outstanding opportunity to study the molecular mechanisms that control adipose mass.
Place, publisher, year, edition, pages
Nature Publishing Group , 2012. Vol. 7, no 3, 606-615 p.
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-76020DOI: 10.1038/nprot.2012.013ISI: 000300948500016OAI: oai:DiVA.org:liu-76020DiVA: diva2:512835
Funding Agencies|Swedish Research Council||Swedish Cancer Foundation||Karolinska Institute Foundation||Karolinska Institute||Torsten Soderbergs Foundation||European Union|222741|European Research Council|250021|2012-03-292012-03-232014-01-21Bibliographically approved