Electrochemical L-Lactic Acid Sensor Based on Immobilized ZnO Nanorods with Lactate Oxidase
2012 (English)In: Sensors, ISSN 1424-8220, Vol. 12, no 3, 2456-2466 p.Article in journal (Refereed) Published
In this work, fabrication of gold coated glass substrate, growth of ZnO nanorods and potentiometric response of lactic acid are explained. The biosensor was developed by immobilizing the lactate oxidase on the ZnO nanorods in combination with glutaraldehyde as a cross linker for lactate oxidase enzyme. The potentiometric technique was applied for the measuring the output (EMF) response of L-lactic acid biosensor. We noticed that the present biosensor has wide linear detection range of concentration from 1 x 10(-4)-1 x 10(0) mM with acceptable sensitivity about 41.33 +/- 1.58 mV/decade. In addition, the proposed biosensor showed fast response time less than 10 s, a good selectivity towards L-lactic acid in presence of common interfering substances such as ascorbic acid, urea, glucose, galactose, magnesium ions and calcium ions. The present biosensor based on immobilized ZnO nanorods with lactate oxidase sustained its stability for more than three weeks.
Place, publisher, year, edition, pages
MDPI , 2012. Vol. 12, no 3, 2456-2466 p.
zinc oxide nanorods, lactate oxidase enzyme, glutaraldehyde, potentiometric nanostructured biosensor, nanodevices
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-76959DOI: 10.3390/s120302456ISI: 000302125000006OAI: oai:DiVA.org:liu-76959DiVA: diva2:524354