Mixed Self-Assembled Monolayers with Deuterated Terminal Anchors for Tethered Lipid Membrane Formation
(English)Manuscript (preprint) (Other academic)
The aim of this study was to develop a stable and flexible self-assembled monolayer (SAM) for tethered lipid bilayer membrane (tBLM) formation that in the future might be employed for mimetics of more complex cell membrane architectures. Our model SAM surface conisted of a mixture of a filling molecule, HS(CH2)15CONHCH2CH2OH, and an anchor molecule, HS(CH2)15CONH(CH2CH2O)6CH2CONH-X, where X is either –(CD2)7CD3 or – (CD2)15CD3. We have prepared and characterized their structure and chemical composition as well as the adsorption of lipids on the SAM by means of ellipsometry, contact angle goniometry, and infrared reflection-absorption spectrometry (IRRAS). The use of deuterated terminal alkyl chains enabled us to establish a correlation between the relative surface density of the anchors and the properties of the lipid bilayers formed via small unilamellar vesicle (SUV) fusion. Further on, we monitored SUV fusion kinetics on the mixed SAM surface using quartz crystal microbalance with dissipative monitoring (QCM-D). The QCM-D analysis revealed that a critical vesicle concentration has to be reached on the SAM for vesicle rupture and bilayer formation.
IdentifiersURN: urn:nbn:se:liu:diva-77050OAI: oai:DiVA.org:liu-77050DiVA: diva2:524722