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The Pharmacological Mechanism of Angiotensin-converting Enzyme Inhibition by Green Tea, Rooibos and Enalaprilat - A Study on Enzyme Kinetics
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences.
2012 (English)In: Phytotherapy Research, ISSN 0951-418X, E-ISSN 1099-1573, Vol. 26, no 4, 517-521 p.Article in journal (Refereed) Published
Abstract [en]

Green tea (Camellia sinensis L.) and Rooibos (Aspalathus linearis Dahlg.) inhibit angiotensin-converting enzyme (ACE) in vitro and in vivo. The ACE inhibitor enalaprilat has been described previously as a competitive inhibitor and sometimes as a non-competitive inhibitor. The aim of this study was to investigate the pharmacological mechanism of ACE inhibition of green tea and Rooibos by enzyme kinetics, and to compare this with enalaprilat. A MichaelisMenten kinetics and LineweaverBurk graph showed mean values of Vmax?=?3.73 mu m and Km?=?0.71 mu m for green tea, of Vmax?=?6.76 mu m and Km?=?0.78 mu m for Rooibos, of Vmax?=?12.54 mu m and Km?=?2.77 mu m for enalaprilat, and of Vmax?=?51.33 mu m and Km?=?9.22 mu m for the PBS control. Incubating serum with green tea or Rooibos saturated with zinc chloride did not change the inhibitory effect. Enalaprilat preincubated with zinc chloride showed a decrease in the inhibitory effect. In conclusion, green tea, Rooibos and enalaprilat seem to inhibit ACE activity using a mixed inhibitor mechanism.

Place, publisher, year, edition, pages
John Wiley and Sons , 2012. Vol. 26, no 4, 517-521 p.
Keyword [en]
Michaelis-Menten, Lineweaver-Burk, flavonoids, tea
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-77101DOI: 10.1002/ptr.3588ISI: 000302620000007OAI: oai:DiVA.org:liu-77101DiVA: diva2:524901
Note
Funding Agencies|Signhild Engkvists Stiftelse||Petrus och Augusta Hedlunds Stiftelse||Available from: 2012-05-04 Created: 2012-05-04 Last updated: 2017-12-07

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Persson, Ingrid A-L

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