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Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury
James Buchanan Brady Urological Institute, Department of Urology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
James Buchanan Brady Urological Institute, Department of Urology, Johns Hopkins Medical Institutions, Baltimore, MD, USA..
James Buchanan Brady Urological Institute, Department of Urology, Johns Hopkins Medical Institutions, Baltimore, MD, USA..
University of Munich, Germany.
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2012 (English)In: International journal of impotence research, ISSN 0955-9930, E-ISSN 1476-5489, Vol. 24, no 2, 84-90 p.Article in journal (Refereed) Published
Abstract [en]

In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48 h in a subset of rats. The MPG was isolated and removed at 48 h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; ∼70% decrease in intracavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury.

Place, publisher, year, edition, pages
2012. Vol. 24, no 2, 84-90 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-77360DOI: 10.1038/ijir.2011.50PubMedID: 21993267OAI: oai:DiVA.org:liu-77360DiVA: diva2:526540
Available from: 2012-05-14 Created: 2012-05-14 Last updated: 2017-12-07

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Hedlund, Petter

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