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Coupling of α1-adrenoceptors to ERK1/2 in the human prostate
University of Munich, Germany.
University of Munich, Germany.
University of Munich, Germany.
University of Munich, Germany.
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2011 (English)In: Urologia internationalis, ISSN 0042-1138, E-ISSN 1423-0399, Vol. 86, no 4, 427-433 p.Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: α1-Adrenoceptors are considered critical for the regulation of prostatic smooth muscle tone. However, previous studies suggested further α1-adrenoceptor functions besides contraction. Here, we investigated whether α1-adrenoceptors in the human prostate may activate extracellular signal-regulated kinases (ERK1/2).

METHODS: Prostate tissues from patients undergoing radical prostatectomy were stimulated in vitro. Activation of ERK1/2 was assessed by Western blot analysis. Expression of ERK1/2 was studied by immunohistochemistry. The effect of ERK1/2 inhibition by U0126 on phenylephrine-induced contraction was studied in organ-bath experiments.

RESULTS: Stimulation of human prostate tissue with noradrenaline (30 μM) or phenylephrine (10 μM) resulted in ERK activation. This was reflected by increased levels of phosphorylated ERK1/2. Expression of ERK1/2 in the prostate was observed in smooth muscle cells. Incubation of prostate tissue with U0126 (30 μM) resulted in ERK1/2 inhibition. Dose-dependent phenylephrine-induced contraction of prostate tissue was not modulated by U0126.

CONCLUSIONS: α1-Adrenoceptors in the human prostate are coupled to ERK1/2. This may partially explain previous observations suggesting a role of α1-adrenoceptors in the regulation of prostate growth.

Place, publisher, year, edition, pages
2011. Vol. 86, no 4, 427-433 p.
Keyword [en]
Prostate hyperplasia, 1-Adrenoceptor, Extracellular signal-regulated kinase, Smooth muscle, Lower urinary tract symptoms, α1-Adrenoceptor blocker
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-77365DOI: 10.1159/000322639PubMedID: 21293101OAI: diva2:526549
Available from: 2012-05-14 Created: 2012-05-14 Last updated: 2012-05-24

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Hedlund, Petter
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Clinical PharmacologyFaculty of Health Sciences
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