liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Expression of cAMP-dependent protein kinase isoforms in the human prostate: functional significance and relation to PDE4
Hannover Medical School, Germany.
Hannover Medical School, Germany.
MorphoSys AG, Martinsried, Germany.
Otto-von-Guericke-University, Magdeburg, Germany.
Show others and affiliations
2010 (English)In: Urology, ISSN 0090-4295, E-ISSN 1527-9995, Vol. 76, no 2, 515.e8-515.e14 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: To investigate the expression of isoforms of the cyclic AMP (cAMP)-dependent protein kinase (cAK) in the transition zone of the human prostate and the functional significance of the enzyme in the control of prostate smooth muscle.

METHODS: Using Western blot analysis and immunohistochemistry, the expression and distribution in the prostate of cAKIalpha, cAKIbeta, cAKIIalpha, and cAKIIbeta in relation to alpha-actin and the phosphodiesterase PDE4 (types A and B) were investigated. The effects of the cAK inhibitor Rp-8-CPT-cAMPS on the reversion of the adrenergic tension of isolated prostate tissue induced by forskolin, rolipram, sodium nitroprusside (SNP), and tadalafil were examined by means of the organ bath technique.

RESULTS: Immunosignals specific for cAKIalpha, cAKIIalpha, and cAKIIbeta were observed in the smooth musculature and glandular structures of the prostate. Double stainings revealed the colocalization of alpha-actin and PDE4 with the cAK isoforms. The expression of the cAK isoforms was confirmed by Western blot analysis. The relaxation of the tension induced by norepinephrine brought about by forskolin, rolipram, SNP, and tadalafil was significantly attenuated by Rp-8-CPT-cAMPS.

CONCLUSIONS: The colocalization of smooth muscle alpha-actin and PDE4 with cAK, as well as the results from the organ bath experiments, provide further evidence for a pivotal role of the cAMP-dependent signaling in the regulation of prostate smooth muscle contractility. Compounds interacting with the cAMP/cAK pathway might represent a new therapeutic avenue to treat symptoms of benign prostatic hyperplasia and lower urinary tract symptomatology.

Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 76, no 2, 515.e8-515.e14 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-77372DOI: 10.1016/j.urology.2010.04.035PubMedID: 20599254OAI: diva2:526557
Available from: 2012-05-14 Created: 2012-05-14 Last updated: 2012-05-24

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hedlund, Petter
By organisation
Clinical PharmacologyFaculty of Health Sciences
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 37 hits
ReferencesLink to record
Permanent link

Direct link