liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
LRRC3B gene is frequently epigenetically inactivated in several epithelial malignancies and inhibits cell growth and replication
Karolinska Institute.
Karolinska Institute.
Karolinska Institute.
Karolinska Institute.
Show others and affiliations
2012 (English)In: Biochimie, ISSN 0300-9084, E-ISSN 1638-6183, Vol. 94, no 5, 1151-1157 p.Article in journal (Refereed) Published
Abstract [en]

Chromosome 3 specific NotI microarrays containing 180 NotI linking clones associated with 188 genes were hybridized to NotI representation probes prepared using matched tumor/normal samples from major epithelial cancers: breast (47 pairs), lung (40 pairs) cervical (43 pairs), kidney (34 pairs of clear cell renal cell carcinoma), colon (24 pairs), ovarian (25 pairs) and prostate (18 pairs). In all tested primary tumors (compared to normal controls) methylation and/or deletions was found. For the first time we showed that the gene LRRC3B was frequently methylated and/or deleted in breast carcinoma - 32% of samples, cervical - 35%, lung - 40%, renal - 35%, ovarian - 28%, colon - 33% and prostate cancer - 44%. To check these results bisulfite sequencing using cloned PCR products with representative two breast, one cervical, two renal, two ovarian and two colon cancer samples was performed. In all cases methylation was confirmed. Expression analysis using RT-qPCR showed that LRRC3B is strongly down-regulated at the latest stages of RCC and ovarian cancers. In addition we showed that LRRC3B exhibit strong cell growth inhibiting activity (more than 95%) in colony formation experiments in vitro in KRC/Y renal cell carcinoma line. All these data suggest that LRRC3B gene could be involved in the process of carcinogenesis as a tumor suppressor gene.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 94, no 5, 1151-1157 p.
Keyword [en]
Cancer, Tumor suppressor gene, NotI microarrays, Methylation, RT-qPCR
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-77531DOI: 10.1016/j.biochi.2012.01.019ISI: 000303292200010OAI: diva2:528604
Funding Agencies|Swedish Cancer Society||Swedish Institute||Swedish Research Council||Karolinska Institute, State|02.740.11.522716.552.11.7034|Russian Ministry of Education and Science||Russian Foundation for Basic Research|10-04-01213-a11-04-00269|Available from: 2012-05-28 Created: 2012-05-22 Last updated: 2012-05-28

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Department of Clinical and Experimental MedicineFaculty of Health Sciences
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 17 hits
ReferencesLink to record
Permanent link

Direct link