Dietary Mustard Seeds (Sinapis alba Linn) Suppress 1,2-Dimethylhydrazine-Induced Immuno-Imbalance and Colonic Carcinogenesis in Rats
2012 (English)In: Nutrition and Cancer, ISSN 0163-5581, E-ISSN 1532-7914, Vol. 64, no 3, 464-472 p.Article in journal (Refereed) Published
In a Wistar rat model, prolonged supplementation of mustard seed (MS) to the diet significantly ameliorates the induction of colorectal carcinomas by 1,2-dimethylhydrazine (DMH). The expression of the splenocyte major histocompatibility complex class I (MHCI) was found significantly enhanced, whereas that of the major histocompatibility complex class II (MHCII) was significantly decreased. Compared to that of control animals, the proportion of spleenic B- and dendritic cells (DC) was amplified in the MS group. The expressions of MHCI, as well as that of MHCII, were increased in DC cells; whereas in B cells, MHCI expression was augmented but that of MHCII moderately decreased. The percentages of CD8+CD28+ and CD4+CD28+ cells were increased in the MS group, while the CD4+CD25+Foxp3+ subset was depressed. Plasma analysis showed that DMH-exposure induced amplified amounts of interleukin (IL)-4, IL-5, IL-10, and transforming growth factor-beta, whereas MS feeding counteracted this effect but enhanced IL-2,IL12p70,IL21, TNF-alpha, and interferon-gamma. In the SW480 colon adenocarcinoma cell-line, the cytotoxicity of spleenic T-cells from MS-fed animals was significantly increased. In the DMH-exposed rats, the expression of perforin in the spleenic T-cells was dramatically decreased, whereas MS abolished this depression. In summary, dietary MS suppresses DMH-induced immuno-imbalance as well as colon carcinogenesis in rats.
Place, publisher, year, edition, pages
Taylor and Francis (Routledge): STM, Behavioural Science and Public Health Titles / Taylor and Francis (Routledge) , 2012. Vol. 64, no 3, 464-472 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-77874DOI: 10.1080/01635581.2012.658948ISI: 000303702600012OAI: oai:DiVA.org:liu-77874DiVA: diva2:529684
Funding Agencies|National Natural Science Foundation of China|81071549|Guangdong Natural Science Foundation|9151040701000025|Guangdong Science and Technology Foundation|2010B060900054|Foundation for Introduction of Innovative R & D Team in Guangdong Province||Nanfang Hospital Foundation|2009B011|Guangdong Talent Person Import Foundation||Southern Medical University Talent Person Import Foundation||China Chunhui Plan||2012-05-312012-05-312012-05-31