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The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles
University of Ottawa.
Karolinska Institute.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
University of Ottawa.
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2012 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 33, no 19, 4947-4956 p.Article in journal (Refereed) Published
Abstract [en]

Spherical 3.5 nm diameter silver nanoparticles (AgNP) stabilized in type I collagen (AgNP@collagen) were prepared in minutes (5-15 min) at room temperature by a photochemical method initiated by UVA irradiation of a water-soluble non-toxic benzoin. This biocomposite was examined to evaluate its biocompatibility and its anti-bacterial properties and showed remarkable properties. Thus, while keratinocytes and fibroblasts were not affected by AgNP@collagen, it was bactericidal against Bacillus megaterium and E. coli but only bacteriostatic against S. epidermidis. In particular, the bactericidal properties displayed by AgNP@collagen were proven to be due to AgNP in AgNP@collagen, rather than to released silver ions, since equimolar concentrations of Ag are about four times less active than AgNP@collagen based on total Ag content. This new biocomposite was stable over a remarkable range of NaCl, phosphate, and 2-(N-morpholino)ethanesulfonic acid concentrations and for over one month at 4 degrees C. Circular dichroism studies show that the conformation of collagen in AgNP@collagen remains intact. Finally, we have compared the properties of AgNP@collagen with a similar biocomposite prepared using alpha-poly-L-Lysine and also with citrate stabilized AgNP; neither of these materials showed comparable biocompatibility, stability, or anti-bacterial activity.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 33, no 19, 4947-4956 p.
Keyword [en]
Silver nano particles, Type-I collagen, Poly-L-Lysine, Cell toxicity, Antimicrobial activity
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-78260DOI: 10.1016/j.biomaterials.2012.03.033ISI: 000303966100018OAI: diva2:531860
Funding Agencies|Natural Sciences and Engineering Research Council (NSERC, Canada)||Available from: 2012-06-08 Created: 2012-06-08 Last updated: 2013-12-17

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