liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles
University of Ottawa.
Karolinska Institute.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
University of Ottawa.
Show others and affiliations
2012 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 33, no 19, 4947-4956 p.Article in journal (Refereed) Published
Abstract [en]

Spherical 3.5 nm diameter silver nanoparticles (AgNP) stabilized in type I collagen (AgNP@collagen) were prepared in minutes (5-15 min) at room temperature by a photochemical method initiated by UVA irradiation of a water-soluble non-toxic benzoin. This biocomposite was examined to evaluate its biocompatibility and its anti-bacterial properties and showed remarkable properties. Thus, while keratinocytes and fibroblasts were not affected by AgNP@collagen, it was bactericidal against Bacillus megaterium and E. coli but only bacteriostatic against S. epidermidis. In particular, the bactericidal properties displayed by AgNP@collagen were proven to be due to AgNP in AgNP@collagen, rather than to released silver ions, since equimolar concentrations of Ag are about four times less active than AgNP@collagen based on total Ag content. This new biocomposite was stable over a remarkable range of NaCl, phosphate, and 2-(N-morpholino)ethanesulfonic acid concentrations and for over one month at 4 degrees C. Circular dichroism studies show that the conformation of collagen in AgNP@collagen remains intact. Finally, we have compared the properties of AgNP@collagen with a similar biocomposite prepared using alpha-poly-L-Lysine and also with citrate stabilized AgNP; neither of these materials showed comparable biocompatibility, stability, or anti-bacterial activity.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 33, no 19, 4947-4956 p.
Keyword [en]
Silver nano particles, Type-I collagen, Poly-L-Lysine, Cell toxicity, Antimicrobial activity
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-78260DOI: 10.1016/j.biomaterials.2012.03.033ISI: 000303966100018OAI: diva2:531860
Funding Agencies|Natural Sciences and Engineering Research Council (NSERC, Canada)||Available from: 2012-06-08 Created: 2012-06-08 Last updated: 2013-12-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Skog, MårtenPolisetti, NareshWickham, AbeniGriffith, May
By organisation
Cell BiologyFaculty of Health SciencesDepartment of Clinical and Experimental MedicineSensor Science and Molecular PhysicsThe Institute of TechnologyOphthalmology
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 243 hits
ReferencesLink to record
Permanent link

Direct link