Two-dimensional gel electrophoresis and mass spectrometry in studies of nanoparticle-protein interactions
2012 (English)In: Gel electrophoresis-Advanced Techniques / [ed] Sameh Magdeldin, Rijeka, Croatia: In Tech , 2012, 1-32 p.Chapter in book (Other academic)
Over the years a number of epidemiological studies have shown that PM from combustion sources such as motor vehicles contributes to respiratory and cardiovascular morbidity and mortality.Especially so do the ultra-fine particles (UFPs) with a diameter less than 0.1 micrometer.UFPs from combustion engines are capable to translocate over the alveolar–capillary barrier. When nano-sized PM (nanoparticles, NP), which are small enough to enter the blood stream, do so they are likely to interact with plasma proteins and this protein-NP interaction will probably affect the fate of and the effects caused by the NPs in the human body. Here, by using a proteomic approach, we present results showing that several proteins indeed are associated to NPs that have in vitro been introduced to human blood plasma.
Place, publisher, year, edition, pages
Rijeka, Croatia: In Tech , 2012. 1-32 p.
Proteins, Nanoparticles, Gelelectrophoresis
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-78397DOI: 10.5772/38085ISBN: 978-953-51-0457-5OAI: oai:DiVA.org:liu-78397DiVA: diva2:532362