Degradation of superparamagnetic iron oxide nanoparticle-induced ferritin by lysosomal cathepsins and related immune response
2012 (English)In: Nanomedicine, ISSN 1743-5889, E-ISSN 1748-6963, Vol. 7, no 5, 705-717 p.Article in journal (Refereed) Published
Aim: To examine the physiological impact of superparamagnetic iron oxide nanoparticles (SPIONs) on cell function and its interaction with oxysterol laden cells. Materials andamp; methods: Intracellular iron was determined by Prussian blue staining. Cellular ferritin, cathepsin L and ferroportin were analyzed by flow cytometry and fluorescence microscopy. Cytokine secretion was determined by ELISA and immunoblotting. Results: In U937 and THP 1 cells, we did not detect any loss of cell viability on SPION loading. Desferrioxamine prevents induction of both ferritin and cathepsin L by SPIONs. Inhibition of lysosomal cathepsins upregulates both endogenous- and SPION-induced ferritin. SPION loading induces membranous ferroportin and incites secretion of ferritin, TNF-alpha and IL-10. 7 beta-hydroxycholesterol exposure reduces SPION uptake by cells. Conclusion: SPION loading results in upregulation of lysosomal cathepsin, membranous ferroportin and ferritin degradation, which is associated with secretion of both pro- and anti-inflammatory cytokines. A reduced SPION uptake by oxysterol-laden cells may lead to a compromised MRI with elevated cathepsins and ferritin.
Place, publisher, year, edition, pages
Future Medicine , 2012. Vol. 7, no 5, 705-717 p.
atherosclerosis, cytokine, degradation, iron, lysosomal, monocyte, nanoparticle
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-78580DOI: 10.2217/nnm.11.148ISI: 000304238300016OAI: oai:DiVA.org:liu-78580DiVA: diva2:534155
Funding Agencies|Swedish Heart Lung Foundation||research fund of Torsten och Ragnar Soderbergs||research fund of Stroke||research fund of Gamla Tjanarinnor||Linkoping University Hospital||2012-06-152012-06-152014-10-30