Graphene-based Biosensor for Intracellular Glucose Measurements
(English)Manuscript (preprint) (Other academic)
In this paper, we report a novel graphene-based glucose micro sensor for measuring intracellular glucose. A borosilicate glass capillary (0.7 um diameter) is coated first with a graphene ink and then with a graphene-enzyme conjugate. The functional groups, presumably on the edge plane of graphene, assist binding with the free amine terminals of the glucose oxidase enzyme to result in a better immobilization. The as-prepared graphene biosensor exhibits a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference is linear over the concentration range of interest (10–1000μM). The measured glucose concentration in human adipocytes by using our graphene based sensor is consistent with reported values of glucose concentration. This device demonstrates a simple technique to measure intracellular glucose concentration.
IdentifiersURN: urn:nbn:se:liu:diva-78679OAI: oai:DiVA.org:liu-78679DiVA: diva2:534724