Linker histones are strongly bound to chromatin in mature avian erythrocytes and loosely bound to chromatin in mature amphibian erythrocytes
(English)Manuscript (preprint) (Other academic)
Linker histones are known to participate in the formation and maintenance of higher order chromatin strnctnres and they are also involved in transcription control. Mature nucleated erythrocytes provide an experimental model to study linker histone binding in cells with highly condensed chromatin and extremely low transcriptional activity. The aim of this study is to investigate linker histone binding in avian and amphibian erythrocytes !mown to contain different subsets of linlcer histones. We used DAPI as an indirect cytochemical probe for the analysis of linker histone affinity for chromatin in situ. We found that linlcer histones were strongly associated to chromatin iu matnre hen erythrocytes which are known to accumulate considerable amounts of H5. Linlcer histones in matnre frog erythrocytes, known to accumulate HIo, showed significantly lower affmity for chromatin. We conclude that linlcer histone affinity is not a key factor for chromatin condensation.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-79457OAI: oai:DiVA.org:liu-79457DiVA: diva2:542462