Transplantation of Autologous Iris Pigment Epithelial Cells To the Subretinal Space: II. Photoreceptor Survival and Consequences of Cellular Multilayers
(English)Manuscript (preprint) (Other academic)
Purpose: To study the effects of transplanted iris pigment epithelial (IPE) cells in the subretinal space on the survival of adjacent photoreceptors.
Methods: An upper iridectomy was made on the right eye of 37 pigmented rabbits. Suspensions of autologous IPE cells were prepared and injected into the subretinal space of the same eye. Follow- up examinations was performed using ophthalmoscopy and calor fundus photography. At 1, 2, 3 and 6 months, respectively, the rabbits were sacrificed and the eyes examined with light and electron microscopy.
Results: On histological examination, the photoreceptor cells were well preserved in grafted areas at 1-3 months. At 6 months, also, the photoreceptors generally disclosed a normal nuclear layer and long outer segments when overlying areas with single cells or clusters of IPE. However, multilayers of transplanted I PE, native RPE cells and macrophages in the subretinal space were frequently associated with photoreceptor damage and nuclear drop out from the outer retinal layer.
Conclusion: In this experimental model, the photoreceptors generally survive adjacent to grafted IPE cells for 6 months. Multilayers of subretinal cells are likely to induce adverse effects on adjacent photoreceptors which is a new finding that requires further investigation.
Retina, IPE transplantation, Autologous, Subretinal space, Melanin pigment distribution, Macrophages, Photoreceptor damage
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-79528OAI: oai:DiVA.org:liu-79528DiVA: diva2:543296