In vitro Lymphocyte Proliferation as Compared to Patch Test Using Gold, Palladium and Nickel
1997 (English)In: International Archives of Allergy and Immunology, ISSN 1018-2438, E-ISSN 1423-0097, Vol. 112, no 3, 212-217 p.Article in journal (Refereed) Published
Background: A conventional lymphocyte transformation test (LTT) was compared to the commercially available MELISA® (memory lymphocyte immuno-stimulation assay), a lymphoproliferative assay that has been suggested to be a valuable instrument for the diagnosis of metal allergy. Sensitivity and specificity of the two assays were calculated using a patch test as a reference method.
Methods: 34 patients were patch-tested for gold sodium thiosulfate, palladium chloride and nickel sulfate, and the lymphocyte proliferation to these metals was tested in vitro using mononuclear cells from peripheral blood.
Results: No significant differences regarding sensitivity and specificity were found between MELISA and conventional LTT. The sensitivity varied between 55 and 95% and the specificity between 17 and 79%.
Conclusions: The low specificity of the two in vitro assays suggests that they are not useful for diagnosis of contact allergy to the metals gold, palladium and nickel, since a large number of false-positive results will be obtained.
Place, publisher, year, edition, pages
1997. Vol. 112, no 3, 212-217 p.
Lymphocytes human, Skin test, Metals
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-79573DOI: 10.1159/000237456OAI: oai:DiVA.org:liu-79573DiVA: diva2:543741