liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Alpha-Dystrobrevin and its associated proteins in human promyelocytic leukemia cells induced to apoptosis
Vilnius University, Lithuania Vilnius Gediminas Technical University, Lithuania .
Vilnius University, Lithuania .
Vilnius University, Lithuania .
Vilnius Gediminas Technical University, Lithuania .
Show others and affiliations
2012 (English)In: Journal of Proteomics, ISSN 1874-3919, Vol. 75, no 11, 3291-3303 p.Article in journal (Refereed) Published
Abstract [en]

Dystrobrevin is a dystrophin-related component of the dystrophin-associated protein complex (DAPC). Using alpha-dystrobrevin as indicator, we aimed to elucidate the interaction network of the DAPC with other proteins during apoptosis of promyelocytic HL-60 cells. The precise role(s) of DBs are not known, but we and others have shown that they play a role in intracellular signal transduction and cellular organization. Apoptosis was induced with etoposide in the absence or presence of Z-VAD to block caspase activity, and we then followed the cellular distribution of alpha-DB and its association with other proteins, using confocal imaging and cell fractions analyses after immune-precipitation with anti-alpha-DB and mass spectrometry. Confocal imaging revealed distinct spatial relocalizations of alpha-DB between the cell membrane, cytosol and nucleus after induction of apoptosis. The expression levels of the identified proteins were evaluated with computer-assisted image analysis of the gels. We thus identified associations with structural and transport proteins (tropomyosin, myosin), membrane (ADAM21, syntrophin), ER-Golgi (TGN51, eIF38) and nuclear (Lamins, ribonucleoprotein C1/C2) proteins. These results suggest that apoptosis-induction in HL-60 cells involves not only classical markers of apoptosis but also a network alpha-DB-associated proteins at the cell membrane, the cytoplasm and nucleus, affecting key cellular transport processes and cellular structure.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 75, no 11, 3291-3303 p.
Keyword [en]
Alpha-DB; Leukemic cells; Apoptosis
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-79700DOI: 10.1016/j.jprot.2012.03.041ISI: 000305718400018OAI: diva2:544096
Available from: 2012-08-13 Created: 2012-08-13 Last updated: 2012-08-13

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Magnusson, Karl-Eric
By organisation
Medical MicrobiologyFaculty of Health Sciences
In the same journal
Journal of Proteomics
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 12 hits
ReferencesLink to record
Permanent link

Direct link