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Assessment of the mosaic structure in the Helicobacter pylori cagA gene 3 -region using an improved polymerase chain reaction-based assay
Linköping University, Department of Clinical and Experimental Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Diagnostics, Department of Clinical Microbiology.
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
2012 (English)In: Diagnostic microbiology and infectious disease, ISSN 0732-8893, E-ISSN 1879-0070, Vol. 73, no 3, 281-283 p.Article in journal (Refereed) Published
Abstract [en]

The mosaic structure of the cagA gene has been suggested to affect Helicobacter pylori CagA-associated pathogenesis. An improved polymerase chain reaction assay allowed for a rapid and detailed molecular analysis of the cagA gene 3-region in a single amplification step, followed by amplicon sequencing using universal M13 and T7 sequencing primers.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 73, no 3, 281-283 p.
Keyword [en]
Molecular genotyping; Amplicon sequencing; cagA EPIYA/T segment; cagA pre-EPIYA/T region; CagA multimerisation motif
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-79684DOI: 10.1016/j.diagmicrobio.2012.03.017ISI: 000305546300018OAI: oai:DiVA.org:liu-79684DiVA: diva2:544273
Available from: 2012-08-14 Created: 2012-08-13 Last updated: 2017-12-07

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Monstein, Hans-JurgRyberg, AnnaKarlsson, Anneli

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Clinical MicrobiologyFaculty of Health SciencesDepartment of Clinical MicrobiologyDepartment of Clinical and Experimental MedicineCell Biology
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