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Detection of p53 gene point mutation using sequence-specific molecularly imprinted PoPD electrode
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
National Institute Mat Science, Japan JSR CREST, Japan .
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-1815-9699
2012 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 35, no 1, 224-229 p.Article in journal (Refereed) Published
Abstract [en]

An amperometric sequence-specific molecularly imprinted single-stranded oligodeoxyribonucleotide (ss-ODN) biosensor was fabricated and characterised in this study. Using ss-ODN as the template and o-phenylenediamine as the functional monomer, the ODN biosensor was fabricated by an electropolymerisation process on an indium-tin oxide (ITO) coated glass substrate. The template ss-ODN was washed out of the ss-ODN/poly(o-phenylenediamine)(PoPD)/ITO electrode using sterilised basic ethanol-water. The resulting ss-ODN imprinted PoPD/ITO electrode was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The amperometric responses, i.e., Delta i as a function of the target ss-ODN concentration was studied. The biosensor using ss-ODN imprinted PoPD/ITO as the working electrode showed a linear Delta current response to the target ss-ODN concentration within the range of 0.01-300 fM. The biosensor showed a sensitivity of 0.62 mu A/fM, with a response time of 14s. The present novel molecularly imprinted ss-ODN biosensor could greatly benefit in terms of cost-effectiveness, storage stability, ultra sensitivity and selectivity together with the potential for improved commercial genetic sensors.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 35, no 1, 224-229 p.
Keyword [en]
Sequence-specific sensor; Genetic recognition; Molecularly imprinted polymer; Oligodeoxyribonucleotide imprinted electrode; Amperometric biosensor
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-79825DOI: 10.1016/j.bios.2012.02.053ISI: 000305036000034OAI: oai:DiVA.org:liu-79825DiVA: diva2:544402
Available from: 2012-08-14 Created: 2012-08-14 Last updated: 2017-12-07

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Tiwari, AshutoshTurner, Anthony

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