Detection of p53 gene point mutation using sequence-specific molecularly imprinted PoPD electrode
2012 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 35, no 1, 224-229 p.Article in journal (Refereed) Published
An amperometric sequence-specific molecularly imprinted single-stranded oligodeoxyribonucleotide (ss-ODN) biosensor was fabricated and characterised in this study. Using ss-ODN as the template and o-phenylenediamine as the functional monomer, the ODN biosensor was fabricated by an electropolymerisation process on an indium-tin oxide (ITO) coated glass substrate. The template ss-ODN was washed out of the ss-ODN/poly(o-phenylenediamine)(PoPD)/ITO electrode using sterilised basic ethanol-water. The resulting ss-ODN imprinted PoPD/ITO electrode was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The amperometric responses, i.e., Delta i as a function of the target ss-ODN concentration was studied. The biosensor using ss-ODN imprinted PoPD/ITO as the working electrode showed a linear Delta current response to the target ss-ODN concentration within the range of 0.01-300 fM. The biosensor showed a sensitivity of 0.62 mu A/fM, with a response time of 14s. The present novel molecularly imprinted ss-ODN biosensor could greatly benefit in terms of cost-effectiveness, storage stability, ultra sensitivity and selectivity together with the potential for improved commercial genetic sensors.
Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 35, no 1, 224-229 p.
Sequence-specific sensor; Genetic recognition; Molecularly imprinted polymer; Oligodeoxyribonucleotide imprinted electrode; Amperometric biosensor
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-79825DOI: 10.1016/j.bios.2012.02.053ISI: 000305036000034OAI: oai:DiVA.org:liu-79825DiVA: diva2:544402