Controlled Assembly of Gold Nanoparticles using De Novo Designed Polypeptide Scaffolds
2008 (English)In: Proceedings SPIE, Vol. 6885, Photonic Biosensing and Microoptics, 2008, 688506-1-688506-8 p.Conference paper (Refereed)
Heterodimerization between designed helix-loop-helix polypeptides was utilized in order to assemble gold nanoparticles on planar substrates. The peptides were designed to fold into four-helix bundles upon dimerization. A Cys-residue in the loop region was used to immobilize one of the complementary peptides on a maleimide containing SAM on planar gold substrates whereas the second peptide was immobilized directly on gold nanoparticles. Introducing the peptide decorated particles over a peptide functionalized surface resulted in particle assembly. Further, citrate stabilized particles were assembled on amino-silane modified glass and silicon substrates. By subsequently introducing peptides and gold nanoparticles, particle-peptide hybrid multi layers could be formed.
Place, publisher, year, edition, pages
2008. 688506-1-688506-8 p.
Heterodimerization, polypeptides, gold nanoparticles, four-helix bundle, helix-loop-helix, self-assembly
National CategoryOther Basic Medicine
IdentifiersURN: urn:nbn:se:liu:diva-15118DOI: 10.1117/12.775806OAI: oai:DiVA.org:liu-15118DiVA: diva2:54441