Radiation and cisplatin sensitivity in head and neck cancer cells with stem cell properties
(English)Manuscript (Other academic)
Existence of cancer stem cells has been verified in tumor cell lines. Here we compare function of lysosomal compartment, as well as cisplatin and radiation sensitivity, in two head and neck squamous cell carcinoma cell lines. LK012 and SqCC/Y1 were found to contain heterogeneous populations of tumor cells, giving rise to holoclones, meroclones and paraclones. Holoclone cells were found to exhibit higher expression of cell surface CD44 as compared to paraclone cells, and their lysosomal compartment was confined to one side of the nucleus as visualized by immunostaining of LAMP-2 (lysosomal associated membrane protein-2) and cathepsin D. In paraclone and CD44 dim cells, lysosomal distribution was evenly located perinuclear. In addition, holoclone cells derived from LK0412 expressed higher levels of the lysosomal protease cathepsin B compared to paraclone cells. When isolated holo- and paraclones were exposed to cisplatin and radiation, holoclone cells from the LK0412 cell line displayed less sensitivity to cisp latin as compared to paraclone cells, while no difference was found in SqCC/Y1 cells. In summary, our results show that the head and neck squamous cell carcinoma cell lines contain subpopulations of cells with cancer stem celllike characteristics. In LK0412 the distinction between different subpopulations was more prominent and holoclone cells showed higher resistant to treatment.
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:liu:diva-15137OAI: oai:DiVA.org:liu-15137DiVA: diva2:54568