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Does healing of an osteochondral defect with or without a periosteal autograft involve a local sprouting of nerve fibres?: An experimental study on the rat patella
Linköping University, Department of Biomedicine and Surgery, Cell biology. Linköping University, Faculty of Health Sciences.
Department of Orthopaedic Surgery, Karolinska Institute, Huddinge University Hospital, Sweden.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Repair of articular cartilage is an important clinical problem. Availability of chondrogenic stem cells has been pointed out as one key factor in cartilage repair and application of periosteal autografts has been used clinically to improve healing. In addition, neuropeptide containing nerve fibres may contribute to healing by stimulating cell proliferation and/or differentiation. In the rat, peptidergic nerve fibres invade the callus formation during fracture healing and peptidergic nerve fibres are abundant in cartilage related connective tissue during skeletal development in young mammals. The purposes of the present study were to evaluate healing of an experimental full thickness osteochondral defect in the rat patella with and without application of a periosteal autograft, and to find out if a local nerve sprouting is part of the healing process. Osteochondral healing was evaluated with a histological score and the presence of nerve fiber profiles in relation to the defect was assessed by protein gene product 9.5 immunohistochemistry. The results showed (i) that osteochondral defects in the rat patella heal spontaneously but incompletely, (ii) that healing consistently is less satisfactory with application of a periosteal autograft than without and (iii) that healing is not accompanied by nerve fibre sprouting.

National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-80302OAI: oai:DiVA.org:liu-80302DiVA: diva2:546475
Available from: 2012-08-23 Created: 2012-08-23 Last updated: 2012-08-23Bibliographically approved
In thesis
1. Sensory nerve fibres, neuropeptides and cartilage: Experimental studies in the rat
Open this publication in new window or tab >>Sensory nerve fibres, neuropeptides and cartilage: Experimental studies in the rat
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

During development, maintenance and repair after injury, reciprocal interactions occur between the peripheral nervous system and the target tissues. In the Papers presented in this thesis, different aspects of such netvetarget influences between peripheral nerve fibres and skeletal tissues dtuing development and repair have been investigated in the rat. Developing rat cartilaginous bone primordia have a richly innervated and vascularised perichondriwn. In addition, larger bones exhibit cartilage canals containing blood vessels and putative sensory nerve fibres. Tills evoked the question if there is a nervous regulation of skeletal development. Denervation of the hind paws of young rats resulted in a deficient length growth but had no influence on the progress of secondary ossification. Since growth is mainly due to events in cartilage, cartilage projecting sensory neurones were identified and examined. Sensory neurones projecting to the rat cartilaginous distal femoral epiphyses were located mainly in the dorsal root ganglia (DRG) L3 and L4 and exhibited small or medium-sized diameters. A large proportion of these neurones contained the neuropeptides CGRP and/or SP. However, application of CGRP to cartilage explants in vitro did not stimulate the chondrocytes in terms of an elevation of the level of cyclic AMP. Another possibility would be that the neuropeptides affect the developmental growth of bone and chondrocytes indirectly via effects on the blood vessels. Experiments .involving tracing as above and eo-culture of labelled DRG neurones and perichondrial cells in combination with immunohistochenllstty or electrophysiology showed that the traced cultured neurones contained CGRP and/or SPin in vivo-like proportions and that most of the cartilage-projecting neurones were proton sensitive, This prompted the suggestion that the nerve fibres in the perichondrium and in cartilage canals might release CGRP and SP in response to local tissue acidosis, thereby promoting tissue homeostasis by monitoring the balance between vascular supply and metabolic load and by influencing angiogenesis and blood flow. Subsequently, possible target influences on the local presence of perichondrial sensory nerve fibres were investigated. Application of inflammation related cytokines (IL-1ß, IL-6 and LIF) affected sensory neurones eo-cultured with perichondrium- or skin-derived fibroblast-like cells in terms of survival and neurite growth. These effects were strongly influenced by the origin of the target cells. Finally, experiments using the adult rat patella showed that osteochondral defects heal spontaneously but incompletely and that healing is not accompanied by an increase of local nerve fibres at the times examined. In conclusion, the present results indicate that cartilagerelated sensory nerve fibres influence skeletal growth, that a high proportion of these neurones contain CGRP and SP, that CGRP does not activate chondrocytes in cartilage slices, that many cartilage related sensory nerve fibres are proton-sensitive· and likely have a vasoregulatory role, that inflammatory mediators have distinct effects on sensory neurones eo-cultivated with perichondrial cells and that healing of an osteochondral defect in the rat patella does not involve a local increase of cartilage-related nerve fibres.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet, 2001. 70 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 712
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-25639 (URN)10014 (Local ID)91-7373-141-1 (ISBN)10014 (Archive number)10014 (OAI)
Public defence
2001-12-14, Berzeliussalen, Universitetssjukhuset, Linköping, 13:00 (Swedish)
Opponent
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2012-08-23Bibliographically approved

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Edoff, Karin

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