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Comprehensive DNA methylation analysis of benign and malignant adrenocortical tumors
Yale University, USA .
Linköping University, Faculty of Health Sciences.
Yale University, USA .
Yale University, USA .
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2012 (English)In: Genes, Chromosomes and Cancer, ISSN 1045-2257, E-ISSN 1098-2264, Vol. 51, no 10, 949-960 p.Article in journal (Refereed) Published
Abstract [en]

The molecular pathogenesis of benign and malignant adrenocortical tumors (ACT) is incompletely clarified. The role of DNA methylation in adrenocortical tumorigenesis has not been analyzed in an unbiased, systematic fashion. Using the Infinium HumanMethylation27 BeadChip, the DNA methylation levels of 27,578 CpG sites were investigated in bisulfite-modified DNA from 6 normal adrenocortical tissue samples, 27 adrenocortical adenomas (ACA), and 15 adrenocortical carcinomas (ACC). Genes involved in cell cycle regulation, apoptosis, and transcriptional regulation of known or putative importance in the development of adrenal tumors showed significant and frequent hypermethylation. Such genes included CDKN2A, GATA4, BCL2, DLEC1, HDAC10, PYCARD, and SCGB3A1/HIN1. Comparing benign versus malignant ACT, a total of 212 CpG islands were identified as significantly hypermethylated in ACC. Gene expression studies of selected hypermethylated genes (CDKN2A, GATA4, DLEC1, HDAC10, PYCARD, SCGB3A1/HIN1) in 6 normal and 16 neoplastic adrenocortical tissues (10 ACA and 6 ACC), displayed reduced gene expression in benign and malignant ACT versus normal adrenocortical tissue. Treatment with 5-aza-2-deoxycytidine of adrenocortical cancer H-295R cells increased expression of the hypermethylated genes CDKN2A, GATA4, DLEC1, HDAC10, PYCARD, and SCGB3A1/HIN1. In conclusion, the current study represents the first unbiased, quantitative, genome-wide study of adrenocortical tumor DNA methylation. Genes with altered DNA methylation patterns were identified of putative importance to benign and malignant adrenocortical tumor development.

Place, publisher, year, edition, pages
Wiley-Blackwell , 2012. Vol. 51, no 10, 949-960 p.
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-80782DOI: 10.1002/gcc.21978ISI: 000307052000005OAI: diva2:548279

Funding Agencies|NIH|UL1 RR024139|Damon Runyon Cancer Research Foundation||Doris Duke Charitable Foundation||

Available from: 2012-08-30 Created: 2012-08-30 Last updated: 2012-08-30

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