liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Growth Factor Release during Preparation and Storage of Platelet Concentrates
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Transfusion Medicine and Clinical Immunology. Linköping University, Department of Biomedicine and Surgery, Cell biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Biomedicine and Surgery, Cell biology. Linköping University, Faculty of Health Sciences.
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Transfusion Medicine and Clinical Immunology. Linköping University, Faculty of Health Sciences.
1995 (English)In: Vox Sanguinis, ISSN 0042-9007, E-ISSN 1423-0410, Vol. 68, no 4, 205-209 p.Article in journal (Refereed) Published
Abstract [en]

The platelet content of platelet-derived growth factor (PDGF), a mitogen stored in the alpha-granules, was studied during preparation and storage of platelet concentrates (PC) and compared to the growth-promoting activity of platelets, β-thromboglobulin (β-TG) and lactate dehydrogenase (LD). We compared PC prepared from platelet-rich plasma (PRP-PC; n= 10) and from buffy coat. Two different pre-preparation storage periods of the buffy coat were used: 4h (BC-PC:4h; n = 10) and 24 h (BC-PC: 24h; n = 5). The platelet content of PDGF and β-TG was measured by a RIA technique and the growth-promoting activity by incorporation of 3H-thymidine in stimulated fibroblasts. The platelet content of PDGF, β-TG and the growth-promoting activity of the platelets decreased in a similar way during preparation and storage of PRP-PC (31 ±2, 35±2 and 33±7%, respectively, at day 5 of storage; mean ± SEM). The release of LD was minor (3.9 ±0.5% at day 5). At day 1 of storage the platelet content of PDGF was significantly better preserved in BC-PC:4h than in BD-PC:24h (88±2 and 81 ±3%, respectively; p = 0.03). Comparing BC-PC:4h and PRP-PC we found a significantly better preservation of PDGF in BC-PC:4h until day 3 of storage (80±2 and 75±1%, respectively at day 3; p = 0.046). In conclusion the preparation of PC according to the PRP method initially induces a higher loss of PDGF, and hence of the growth-promoting activity, than the BC method.

Place, publisher, year, edition, pages
1995. Vol. 68, no 4, 205-209 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-81056DOI: 10.1111/j.1423-0410.1995.tb02573.xOAI: oai:DiVA.org:liu-81056DiVA: diva2:550073
Available from: 2012-09-06 Created: 2012-09-06 Last updated: 2017-12-07Bibliographically approved
In thesis
1. A study of factors influencing the quality of blood products during preparation, storage and filtration
Open this publication in new window or tab >>A study of factors influencing the quality of blood products during preparation, storage and filtration
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The quality of a cell concentrate to be transfused is dependent on the method of preparation and the storage conditions of the blood products. The aim of this study was to determine, compare and evaluate factors influencing the quality of platelet and erythrocyte concentrates. The influence of the method of preparation on platelet concentrates from whole blood and on leukocyte depletion by filtration of erythrocyte concentrates was studied. In addition, the influence of storage on leukocyte depletion by filtration of platelet and erythrocyte concentrates was investigated.

The method of preparation of platelet concentrates from whole blood influenced the release from the platelet α-granules. A significant increase in the release was found in the concentrates prepared from platelet-rich plasma compared with buffy coat. If the buffy coat was allowed to rest for <4 hours before centrifugation, this difference was significant until day 3 of storage. The ability of platelets to stimulate the growth of fibroblasts followed a similar course and decreased during preparation and storage.

The method of preparation of erythrocyte concentrates was shown to influence the outcome of leukocyte depletion by filtration. When hard spun, buffy coat depleted, concentrates were used, the number of leukocytes found in the filtrate was significantly higher compared with units that had been supplemented with an additional 5 or 10 ml of plasma. The flow rate during filtration and temperature of the unit was also shown to have an influence on the outcome on the number of leukocytes post filtration.

The storage time of both erythrocyte and platelet concentrates resulted in significant differences in the number ofleukocytes found after leukocyte depletion by filtration. A short storage time of erythrocyte concentrates was found to give a higher number of leukocytes after filtration compared with a longer storage time. This was in contrast to platelet concentrates where a filtration just after preparation, i.e. no storage time, gave better leukocyte depletion compared with 5 days of storage.

The distribution ofleukocyte subsets was also changed significantly by filtration. Comparing the pre- and post-filtration percentages of subsets in platelet concentrates, we found a lower percentage of T-lymphocytes and a higher percentage of B-lymphocytes and monocytes post filtration. In conclusion, the method of preparation of cell concentrates and the storage time have a substantial impact on the properties of the final product. Standardized and controlled procedures are of great importance in making optimal blood products.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2001. 64 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 667
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-28599 (URN)13753 (Local ID)91-7219-960-1 (ISBN)13753 (Archive number)13753 (OAI)
Public defence
2001-04-21, Elsa Brännströmssalen, Universitetssjukhuset, Linköping, 10:00 (English)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-09-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Wasteson, ÅkeBerlin, Gösta

Search in DiVA

By author/editor
Wasteson, ÅkeBerlin, Gösta
By organisation
Department of Transfusion Medicine and Clinical ImmunologyCell biologyFaculty of Health Sciences
In the same journal
Vox Sanguinis
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 64 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf