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Inadequate white cell reduction by bedside filtration of red cell concentrates
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Transfusion Medicine and Clinical Immunology. Linköping University, Faculty of Health Sciences.
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Transfusion Medicine and Clinical Immunology. Linköping University, Faculty of Health Sciences.
1994 (English)In: Transfusion, ISSN 0041-1132, E-ISSN 1537-2995, Vol. 34, no 9, 765-768 p.Article in journal (Refereed) Published
Abstract [en]

Background: White cell filtration of red cell concentrates is often performed at the bedside, in the ward, with the filter inserted in the blood administration line. The aim of this study was to evaluate the efficiency of this filtration method and compare it to filtration in the blood bank.

Study Design and Methods: One-day-old, buffy coat-reduced, hard-packed red cell concentrates in saline-adenine-glucose-mannitol solution were filtered through different filters designed for bedside or laboratory use. With filters designed for bedside use, filtration of red cells was performed under laboratory conditions at fast flow (10 min) or under bedside conditions at slow flow (2 hours). The remaining white cells were counted microscopically. Filters designed for laboratory use were evaluated at fast flow, and the number of contaminating white cells was counted by flow cytometry.

Results: With bedside fllters, a significantly higher contamination of white cells was found In the units filtered at slow flow than at fast flow, regardless of the filter used. The number of units with >5 x 106 white cells was 52 (78%) of 67 filtered at slow flow compared to 11 (23%) of 47 at fast flow, all filters taken together. This difference in white cell contamination was mainly due to an increase of polymorphonuclear cells in the red cell concentrates filtered at slow flow. With filters designed for laboratory use, 0 to 2 percent of units (n = 1448) were contaminated with >5 x 106 white cells.

Conclusion: Bedside filtration for white cell reduction at slow flow is inefficient for 1-day-old, buffy coat-reduced red cell concentrates.

Place, publisher, year, edition, pages
1994. Vol. 34, no 9, 765-768 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-81057DOI: 10.1046/j.1537-2995.1994.34994378276.xOAI: oai:DiVA.org:liu-81057DiVA: diva2:550081
Available from: 2012-09-06 Created: 2012-09-06 Last updated: 2017-12-07Bibliographically approved
In thesis
1. A study of factors influencing the quality of blood products during preparation, storage and filtration
Open this publication in new window or tab >>A study of factors influencing the quality of blood products during preparation, storage and filtration
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The quality of a cell concentrate to be transfused is dependent on the method of preparation and the storage conditions of the blood products. The aim of this study was to determine, compare and evaluate factors influencing the quality of platelet and erythrocyte concentrates. The influence of the method of preparation on platelet concentrates from whole blood and on leukocyte depletion by filtration of erythrocyte concentrates was studied. In addition, the influence of storage on leukocyte depletion by filtration of platelet and erythrocyte concentrates was investigated.

The method of preparation of platelet concentrates from whole blood influenced the release from the platelet α-granules. A significant increase in the release was found in the concentrates prepared from platelet-rich plasma compared with buffy coat. If the buffy coat was allowed to rest for <4 hours before centrifugation, this difference was significant until day 3 of storage. The ability of platelets to stimulate the growth of fibroblasts followed a similar course and decreased during preparation and storage.

The method of preparation of erythrocyte concentrates was shown to influence the outcome of leukocyte depletion by filtration. When hard spun, buffy coat depleted, concentrates were used, the number of leukocytes found in the filtrate was significantly higher compared with units that had been supplemented with an additional 5 or 10 ml of plasma. The flow rate during filtration and temperature of the unit was also shown to have an influence on the outcome on the number of leukocytes post filtration.

The storage time of both erythrocyte and platelet concentrates resulted in significant differences in the number ofleukocytes found after leukocyte depletion by filtration. A short storage time of erythrocyte concentrates was found to give a higher number of leukocytes after filtration compared with a longer storage time. This was in contrast to platelet concentrates where a filtration just after preparation, i.e. no storage time, gave better leukocyte depletion compared with 5 days of storage.

The distribution ofleukocyte subsets was also changed significantly by filtration. Comparing the pre- and post-filtration percentages of subsets in platelet concentrates, we found a lower percentage of T-lymphocytes and a higher percentage of B-lymphocytes and monocytes post filtration. In conclusion, the method of preparation of cell concentrates and the storage time have a substantial impact on the properties of the final product. Standardized and controlled procedures are of great importance in making optimal blood products.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2001. 64 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 667
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-28599 (URN)13753 (Local ID)91-7219-960-1 (ISBN)13753 (Archive number)13753 (OAI)
Public defence
2001-04-21, Elsa Brännströmssalen, Universitetssjukhuset, Linköping, 10:00 (English)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-09-06Bibliographically approved

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