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Factors influencing white cell removal from red cell concentrates by filtration
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Transfusion Medicine and Clinical Immunology. Linköping University, Faculty of Health Sciences.
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Transfusion Medicine and Clinical Immunology. Linköping University, Faculty of Health Sciences.
1996 (English)In: Transfusion, ISSN 0041-1132, E-ISSN 1537-2995, Vol. 36, no 8, 714-718 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The preparation of blood components by hard centrifugation results in red cell concentrates with a small amount of plasma. The influence of various plasma factors, temperature, and storage time on white cell reduction by filtration was studied. STUDY

DESIGN AND METHODS: Red cell concentrates were suspended in 100 mL of saline- adenine-glucose-mannitol (SAGMAN) solution or in SAGMAN solution in which 5 or 10 mL had been replaced with an equal amount of fresh plasma, albumin (4%), or heat-inactivated plasma. After overnight storage at 4 degrees C, filtration at a slow flow rate (2 hours) was performed. The effect of temperature was studied by filtration at 4 degrees C and 37 degrees C. To study the influence of storage time, red cell concentrates were stored for 4 to 8 hours or 14 to 20 hours at 4 degrees C and filtered through another model of filter. The number of white cells was counted microscopically or by flow cytometry.

RESULTS: When 5 or 10 mL of plasma was added, a significantly smaller number of white cells were found after filtration than were found in the SAGMAN control (the median difference between pairs: 23.6 × 10(6) for 5 mL [p = 0.006] and 14.9 × 10(6) for 10 mL [p = 0.003]). The number of white cells was significantly higher with 10 mL of albumin than with 10 mL of plasma (difference, 15.0 × 10(6); p = 0.006). When heat-inactivated plasma was used, the number of white cells was significantly lower than when fresh plasma was used (difference, 0.3 × 10(6); p = 0.009). Filtration at 37 degrees C resulted in a 64-percent reduction in white cells and that at 4 degrees C led to a 99.7-percent reduction (p = 0.006). When the second filter was used, a slight but significantly lower number of white cells was found in the red cell concentrate stored for 14 to 20 hours than in that stored for 4 to 8 hours (difference, 0.03 × 10(6); p < 0.001).

CONCLUSION: The amount of plasma in the red cell concentrate and the storage time and temperature are important factors in the outcome of white cell reduction by filtration. The effect of plasma does not seem to be due to a general influence of protein or to the activity of complement or fibrinogen.

Place, publisher, year, edition, pages
1996. Vol. 36, no 8, 714-718 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-81058DOI: 10.1046/j.1537-2995.1996.36896374375.xOAI: oai:DiVA.org:liu-81058DiVA: diva2:550089
Available from: 2012-09-06 Created: 2012-09-06 Last updated: 2017-12-07Bibliographically approved
In thesis
1. A study of factors influencing the quality of blood products during preparation, storage and filtration
Open this publication in new window or tab >>A study of factors influencing the quality of blood products during preparation, storage and filtration
2001 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The quality of a cell concentrate to be transfused is dependent on the method of preparation and the storage conditions of the blood products. The aim of this study was to determine, compare and evaluate factors influencing the quality of platelet and erythrocyte concentrates. The influence of the method of preparation on platelet concentrates from whole blood and on leukocyte depletion by filtration of erythrocyte concentrates was studied. In addition, the influence of storage on leukocyte depletion by filtration of platelet and erythrocyte concentrates was investigated.

The method of preparation of platelet concentrates from whole blood influenced the release from the platelet α-granules. A significant increase in the release was found in the concentrates prepared from platelet-rich plasma compared with buffy coat. If the buffy coat was allowed to rest for <4 hours before centrifugation, this difference was significant until day 3 of storage. The ability of platelets to stimulate the growth of fibroblasts followed a similar course and decreased during preparation and storage.

The method of preparation of erythrocyte concentrates was shown to influence the outcome of leukocyte depletion by filtration. When hard spun, buffy coat depleted, concentrates were used, the number of leukocytes found in the filtrate was significantly higher compared with units that had been supplemented with an additional 5 or 10 ml of plasma. The flow rate during filtration and temperature of the unit was also shown to have an influence on the outcome on the number of leukocytes post filtration.

The storage time of both erythrocyte and platelet concentrates resulted in significant differences in the number ofleukocytes found after leukocyte depletion by filtration. A short storage time of erythrocyte concentrates was found to give a higher number of leukocytes after filtration compared with a longer storage time. This was in contrast to platelet concentrates where a filtration just after preparation, i.e. no storage time, gave better leukocyte depletion compared with 5 days of storage.

The distribution ofleukocyte subsets was also changed significantly by filtration. Comparing the pre- and post-filtration percentages of subsets in platelet concentrates, we found a lower percentage of T-lymphocytes and a higher percentage of B-lymphocytes and monocytes post filtration. In conclusion, the method of preparation of cell concentrates and the storage time have a substantial impact on the properties of the final product. Standardized and controlled procedures are of great importance in making optimal blood products.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2001. 64 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 667
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-28599 (URN)13753 (Local ID)91-7219-960-1 (ISBN)13753 (Archive number)13753 (OAI)
Public defence
2001-04-21, Elsa Brännströmssalen, Universitetssjukhuset, Linköping, 10:00 (English)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-09-06Bibliographically approved

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