Mutagenicity from neutrophils after challenge with Helicobacter pylori and bile
1997 (English)In: European Journal of Surgery, ISSN 1102-4151, Vol. 163, no 10, 753-759 p.Article in journal (Refereed) Published
OBJECTIVE: To study some mechanisms involved in Helicobacter pylori (H. pylori)-induced gastric carcinogenesis.
DESIGN: In vitro study.
SETTING: Medical centre hospital, Sweden.
INTERVENTIONS: Mutagenicity in Ames' test of neutrophils challenged for 2 hours or more by two different strains of H. pylori. One strain designated NCTC 11637 by the National College of Type Cultures activated neutrophils to an oxidative burst and producing vacuolating cytotoxin, the other strain C-7050 lacked these abilities. Mutagenicity was also studied with sterile human gall bladder bile alone added to neutrophils or in combination with both neutrophils and H. pylori.
RESULTS: There was no increase in the number of revertants with the crude suspension or the supernatant of neutrophils challenged for 1 hour or less with H. pylori, bile, or the combination of both. However, in 5 out of 19 experiments there was significant mutagenicity after challenge of neutrophils for 2 hours or more with either strain of H. pylori, bile, or the combination of the two. The strongest mutagenicity was obtained after challenge over night (18 hours) with the combination of H. pylori and bile.
CONCLUSION: Mutagenicity occurs when neutrophils are challenged with H. pylori and bile. Factors other than reactive oxygen metabolites seem to be responsible.
Place, publisher, year, edition, pages
1997. Vol. 163, no 10, 753-759 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-81126PubMedID: 9373226OAI: oai:DiVA.org:liu-81126DiVA: diva2:550678