Helicobacter pylori is not mutagenic in Ames test
2001 (English)In: Cancer Research, Therapy and Control, ISSN 1064-0525, Vol. 11, no 2, 167-173 p.Article in journal (Refereed) Published
Problem: Helicobacter pylori is classified as a class 1 carcinogen. However, the role of Helicobacter pylori in the initiation and promotion of gastric cancer is not known.
Methods: Mutagenicity has been measured in pure cultures of the cytotoxic Helicobacter pylori strains Ca 18 and NCTC 11637 and in extracts of Helicobacter pylori infected mouse stomach mucosa using the Salmonella mutagenicity test (Ames test). Chemiluminiscence assay was also used to verify the bacterial effect on inflammatory leukocytes.
Results: All Helicobacter strains studied induced a strong oxidative burst of polymorphonuclear leukocytes (PMNL). No direct or indirect mutagenic activity was found.
Conclusions: This confirm the concept that Helicobacter pylori is neither genotoxic per se nor indirectly by inducing mutagenic activity in gastric mucosa. Other mechanisms seems to play a role in the complex, multistep, and multifactorial process of gastric carcinogenesis.
Place, publisher, year, edition, pages
2001. Vol. 11, no 2, 167-173 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-81127OAI: oai:DiVA.org:liu-81127DiVA: diva2:550682