Cyclooxygenase-2 and Bcl-2 expression in the stomach mucosa of Wistar rats exposed to Helicobacter pylori, MNNG and bile
(English)Manuscript (preprint) (Other academic)
Cyclooxygenase-2 (COX-2) and Bcl-2 may mirror different stages of gastric carcinogenesis, but the underlying mechanisms are not known. Thus the relation of COX-2 and Bcl-2 to known cell kinetics were studied in the glandular stomach mucosa of 104 Wistar rats given combinationsof H. pylori, MNNG (methyl-N'nitro-N-nitrosoguanidine) and bile.
COX-2 expression, Bcl-2 and cell proliferation (Ki-67) in antral and corpus mucosa were determined immunohistochemically. Apoptotic cells were detected by using terminal uridine deoxynucleotidyl nick end labeling technique.
Expression of COX-2 was found in the lower portion of glandular corpus epithelium and Bcl-2 positivity was mainly seen in the proliferative zone ofboth antmm and corpus mucosa. COX-2 in histologically normal-appearing corpus mucosa was associated with cell proliferation, atrophy and intestinal metaplasia in antmm and with Bcl-2 expression in corpus mucosa. No correlation was found between apoptosis and COX-2 or Bcl-2 expression. MNNG but not H. pylori significantly increased COX-2 in corpus mucosa. H.pylori, however, promoted the COX-2 expression in corpus when bile was added and Bcl-2 expression in antrum. These findings may indicate an early phase of gastric neoplasia in a state of increased cell proliferation.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-81130OAI: oai:DiVA.org:liu-81130DiVA: diva2:550702