Hepatocyte growth factor accelerates restitution of damaged mouse melanocyte monolayer in vitro
(English)Manuscript (preprint) (Other academic)
In a recent pilot study we observed enhanced wound repair properties in chronic leg ulcers after HGF administration. The aim of the present study was to assess the mechanism of HGF in the wound-healing process by an in vitro study of HGF on a transformed mouse skin epithelial cell line (CCL-53. 1) that expresses the HGF receptor, c-Met. HGF did not enhance cell proliferation, judged by [3H}thymidine incorporation. The restitution of injured epithelial cells was significantly increased by HGF. This effect was dose-dependant, beginning at low concentrations (1ng/ml) of HGF, and was inhibited by heparin. HGF enhanced migration of epithelial cells compared to untreated controls. The actin structure was changed by HGF, with less F-actin in the dense peripheral structures compared to untreated cells. Corresponding morphological changes in cells were found showing spread morphology with smaller intracellular spaces after HGF addition. We conclude that HGF may contribute to restitution of the damaged mouse epithelial cell monolayer by morphologic changes and enhanced motility of cells towards the injured area.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-81260OAI: oai:DiVA.org:liu-81260DiVA: diva2:551253