Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins
1994 (English)In: British Journal of Cancer, ISSN 0007-0920, E-ISSN 1532-1827, Vol. 70, no 5, 873-879 p.Article in journal (Refereed) Published
Laser-induced fluorescence (LIF) was used to characterise the localisation of an intravenously administered trimethylated carotenoporphyrin [CP(Me)3] and a trimethoxylated carotenoporphyrin [CP(OMe)3] in an intramuscularly transplanted malignant tumour (MS-2 fibrosarcoma) and healthy muscle in female Balb/c mice, 3, 24, 48 and 96 h post injection. The fluorescence was induced with a dye laser pumped by a nitrogen laser, emitting light at 425 nm. The fluorescence spectra were recorded in the region 455-760 nm using a polychromator equipped with an image-intensified CCD camera. The tumour/peritumoral muscle ratio was about 5:1 for CP(Me)3 and about 6:1 for CP(OMe)3 in terms of the background-free fluorescence intensity, which peaked at about 655 nm. By including the endogenous tissue fluorescence, the contrast was further enhanced by a factor of approximately 2.
Place, publisher, year, edition, pages
1994. Vol. 70, no 5, 873-879 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-81355DOI: 10.1038/bjc.1994.413OAI: oai:DiVA.org:liu-81355DiVA: diva2:551913