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Laser-induced fluorescence studies of the biodistribution of carotenoporphyrins in mice
Lund University Medical Laser Center, Lund Institute of Technology, Lund, Sweden.
Lund University Medical Laser Center and Department of Physics, Lund Institute of Technology, Lund, Sweden.
Lund University Medical Laser Center, Lund Institute of Technology and the Department of Oncology, Lund University Hospital, Lund, Sweden.
Lund University Medical Laser Center and Department of Physics, Lund Institute of Technology, Lund, Sweden.
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1997 (English)In: British Journal of Cancer, ISSN 0007-0920, E-ISSN 1532-1827, Vol. 76, no 3, 355-364 p.Article in journal (Refereed) Published
Abstract [en]

The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

Place, publisher, year, edition, pages
1997. Vol. 76, no 3, 355-364 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-81356DOI: 10.1038/bjc.1997.390OAI: oai:DiVA.org:liu-81356DiVA: diva2:551916
Available from: 2012-09-12 Created: 2012-09-12 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Photon migration in tissue: laser induced fluorescence for cancer diagnostics and influence of optical properties on microvascular Doppler spectroscopy
Open this publication in new window or tab >>Photon migration in tissue: laser induced fluorescence for cancer diagnostics and influence of optical properties on microvascular Doppler spectroscopy
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Laser induced flourescence (LIF) is an "optical biopsy" method, based on the selective accumulation of fluorophores in neoplastic tissue. Two recently developed, non-photosensitizing tumor seeking carotenoporphyrins were assessed regarding tumor selectivity, and biodistribution, in experimental animals. A tumor to peritumoral ratio of 5-6:1 was seen in the background free substance related fluorescence in vivo, as well as ex vivo. Cerebral cortex and skeletal muscle displayed a low, and liver a high substance related fluorescence.

Laser Doppler flowmetry (LDF) is based on the spectral broadening of monochromatic light, that interacts with moving red blood cells in tissue. The power spectral density of the backscattered light can be processed to yield an estimate of microvascular tissue perlusion. Using a Monte Carlo simulation model of human skin, it is demonstt·ated that for a particular light delivery/detection arrangement, Doppler shifted photons that originate from the central core and peripheral parts of blood vessels of physiological dimensions, both contribute to the detected signal. Further, more than 10 times as many photons will interact with the superficial as with the deep vascular plexus. However, due to greater velocities and concentrations of the moving scatterers, the profound circulation still may yield a greater contribution to the LDF perfusion estimate.

A multiple polynomial regression method for prediction of photon pathlength and optical properties in tissue, at surlace source detector separations up to two millimeters, was developed. Using the diffuse, backscattered reflectance profile from an array of optical sensors as predictors in the model resulted in root-meansquare errors of less than three per cent for the estimated pathlength. Caucasian human skin displayed a maximum in vivo variation of ~35 % in the photon pathlength between individuals in similar locations, and within individuals comparing fingertip and forearm skin, as a result of varying optical properties.

Assuming a homogenous tissue perlusion, the pathlength variations will induce a corresponding variation in the LDF petfusion signal, which can be compensated for by linearization and pathlength normalization, making intra- and interindividual comparisons of the LDF perfusion estimate feasible.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet, 2002. 123 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 735
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-29435 (URN)14781 (Local ID)91-7373-179-X (ISBN)14781 (Archive number)14781 (OAI)
Public defence
2002-05-31, Administrationsbyggnadens aula, Universitetssjukhuset, Linköping, 13:15 (Swedish)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-10-08Bibliographically approved

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