Can a one-layer optical skin model including melanin and inhomogeneously distributed blood explain spatially resolved diffuse reflectance spectra?
2011 (English)In: Optical Tomography and Spectroscopy of Tissue IX / [ed] Robert R. Alfano; Bruce J. Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva M. Sevick-Muraca, SPIE - International Society for Optical Engineering, 2011, Vol. 7896, 78962Y-78962Y-9 p.Conference paper (Other academic)
Model based analysis of calibrated diffuse reflectance spectroscopy can be used for determining oxygenation and concentration of skin chromophores. This study aimed at assessing the effect of including melanin in addition to hemoglobin (Hb) as chromophores and compensating for inhomogeneously distributed blood (vessel packaging), in a single-layer skin model. Spectra from four humans were collected during different provocations using a twochannel fiber optic probe with source-detector separations 0.4 and 1.2 mm. Absolute calibrated spectra using data from either a single distance or both distances were analyzed using inverse Monte Carlo for light transport and Levenberg-Marquardt for non-linear fitting. The model fitting was excellent using a single distance. However, the estimated model failed to explain spectra from the other distance. The two-distance model did not fit the data well at either distance. Model fitting was significantly improved including melanin and vessel packaging. The most prominent effect when fitting data from the larger separation compared to the smaller separation was a different light scattering decay with wavelength, while the tissue fraction of Hb and saturation were similar. For modeling spectra at both distances, we propose using either a multi-layer skin model or a more advanced model for the scattering phase function.
Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2011. Vol. 7896, 78962Y-78962Y-9 p.
, Proceedings of SPIE - International Society for Optical Engineering, ISSN 0277-786X, EISSN 1996-756X ; 7896
diffuse reflectance spectroscopy, light transport, Monte Carlso simulation, tissue moedeling, vessel packaging, skin
Medical Laboratory and Measurements Technologies
IdentifiersURN: urn:nbn:se:liu:diva-81240DOI: 10.1117/12.873134OAI: oai:DiVA.org:liu-81240DiVA: diva2:552634
Optical Tomography and Spectroscopy of Tissue IX Conference, San Francisco, California, January 22, 2011