A single genotype of Legionella pneumophila serogroup 1 found in 96% of isolates from a hospital and minucipal water distribution system over a 12-year period
(English)Manuscript (preprint) (Other academic)
The genotypic distribution of Legionella pneumophila serogroup 1 was investigated in the water distribution system of a 450 bed Swedish hospital and the surrounding community. A single genotype identified by amplified fragment length polymorphism (AFLP) analysis, was found in all 34 hospital isolates and in 18/20 (90%) community isolates over a 12-year surveillance period. All enviromnental isolates were either monoclonal antibody subtypes Benidorm or Bellingham. In a geographic comparison, the hospital genotype was also identified in two out of six Swedish hospitals; both located within 100 km of the studied community. In all, 70 isolates from seven Swedish communities clustered in four groups, each also containing one AFLP type as defined by the European Working Group on Legionella Infections (EWGLI). It was concluded that a single Legionella pneumophila serogroup 1 genotype may colonise a large water distribution system over a long period of time, and that certain clones seem to be widely spread in the environment. Results frorn molecular typing of isolates originating from a limited geographical area must, therefore, be interpreted cautiously in epidemiological investigations of Legionnaires' disease.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-84313OAI: oai:DiVA.org:liu-84313DiVA: diva2:558681