High concentration but low biological activity of hepatocyte growth factor in patients with chronic renal failure
2012 (English)In: Advances in Bioscience and Biotechnology, ISSN 2156-8456, EISSN 2156-8502, Vol. 3, no 4, 516-523 p.Article in journal (Refereed) Published
Hepatocyte growth factor (HGF) is a renotropic, an- tifibrotic and regenerative factor with cytoprotective effects that is produced by mesenchymal cells and shows high affinity to components of extra cellular matrix, such as heparan sulphate proteoglycan (HS- PG), in healthy. Patients with chronic renal failure (CRF) suffer from a chronic inflammatory disorder. In order to assess the underlying mechanisms for de- velopment of CRF we aimed to assess the amounts and affinity of HGF in this patient group. ELISA, western blot and surface plasmon resonance (SPR) were used to study HGF in blood samples, as well as in isolated neutrophils, in CRF patients compared to healthy controls. Patients with CRF showed higher HGF levels in serum (P < 0.0001), but decreased af- finity to HSPG (P < 0.0001). Addition of protease in-hibitors decreased the difference between patients with CRF compared to healthy individuals. HGF with potent regenerative function during injury lacks af-finity to HSPG in patients with CRF that may depend on production of proteases from activated immune cells. This information might be used to highlight un-derlying mechanisms for chronicity and leading to new strategies for treatment of chronic injuries.
Place, publisher, year, edition, pages
Scientific Research Publishing, 2012. Vol. 3, no 4, 516-523 p.
Chronic Renal Failure; Hepatocyte Growth Factor; Biological Activity; Neutrophils
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-84491DOI: 10.4236/abb.2012.324068OAI: oai:DiVA.org:liu-84491DiVA: diva2:559671