Cloning and characterization of 5'-end alternatively spliced human cholecystokinin-B receptor mRNAs
1998 (English)In: Receptors and Channels, ISSN 1060-6823, Vol. 6, no 3, 165-177 p.Article in journal (Refereed) Published
We report here the cloning and characterization of a 5'-end alternatively spliced human cholecystokinin-B (CCK-B) receptor mRNA. The 5'-end of this CCK-B receptor transcript (termed CCK-BRtx) consisted of exon Ia, present in the ordinary full-length CCK-B receptor mRNA (CCK-BRwt), and exon Ib, present in a previously described 5'-end alternatively spliced CCK-B receptor mRNA (CCK-BRt). A short open reading frame preceded the AUG translation initiation codon of the CCK-BRtx. Transfection of COS-7 cells with the CCK-BRtx or CCK-BRt cDNAs did not lead to the appearance of peptidergic and non-peptidergic binding sites. Cell free in vitro translation yielded proteins of approximately 44 kDa (CCK-B receptor) and 40 kDa (CCK-BRt receptor) whereas no 40 kDa product was detected from the cloned CCK-BRtx cDNA. Instead, a protein product of approximately 9 kDa was visualized, the size corresponding to the predicted protein encoded by the short open reading frame. The alternatively spliced CCK-B receptor transcripts were concomitantly expressed with the ordinary full-length CCK-B receptor mRNA in the brain, pancreas, and stomach. The possibility that such transcripts are translated in vivo into truncated CCK-B receptors is discussed.
Place, publisher, year, edition, pages
1998. Vol. 6, no 3, 165-177 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-84493PubMedID: 10100325OAI: oai:DiVA.org:liu-84493DiVA: diva2:559693