Quantification of alpha-synuclein in cerebrospinal fluid
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
To date there is no accepted clinical diagnostic test for Parkinson's disease (PD) based on biochemical analyses of blood or cerebrospinal uid. Currently, diagnosis, measurement of disease progression and response to therapeutic intervention are based on clinical observation, but the rst neuronal dysfunction precede the earliest recognition of symptom by at least 5 - 10 years. A potential diagnostic biomarker is oligomeric alpha-synuclein which in recent papers have reported a signicant quantitative dierence between PD and controls. In this master thesis, a method for measuring oligomeric levels of alpha-synuclein is presented together with a monomeric measuring commercial kit used to measure alpha-synuclein in a preclinical model of PD. A signicant dierence of monomeric levels could be detected between two weeks and four weeks post injection of a vector containing the gene for human alpha-synuclein, no signicant dierence between four and eight weeks was found.
Place, publisher, year, edition, pages
2012. , 40 p.
alpha-synuclein parkinson cerebrospinal fluid biomarker
IdentifiersURN: urn:nbn:se:liu:diva-84598ISRN: LiTH-IFM-A-EX--12/2591--SEOAI: oai:DiVA.org:liu-84598DiVA: diva2:560511
Subject / course
Höglund, Kina, DoktorSvensson, Samuel, Professor
Hammarström, Per, Professor