Advances in freeze-fracture replication for assessing immune interactions between Helicobacter pylori and human neurophils
(English)Manuscript (preprint) (Other academic)
A freeze-fracture replica labeling method adapted for studies of bacterial envelopes was recently introduced. This report describes a further development of this detergent-digested freeze-fracture replica labeling technique, enabling direct visualization of bacteria-host interaction, specifically between the gastric pathogen Helicobacter pylori and human neutrophils. The phagocytic process performed by the neutrophilic leukocytes represents a crucial element of the host defense system against invading microorganisms. The present methodology can be used as a tool that allows characterization of the molecular and ultrastructural events that take place between pathogenic microbes and professional phagocytes.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-84621OAI: oai:DiVA.org:liu-84621DiVA: diva2:560769