N-CoR interacts directly with the DNA sequence ATNNTNCTC
(English)Manuscript (preprint) (Other academic)
N-CoR (nuclear receptor corepressor) and SMRT (silencing mediator of thyroid and retinoid hormone receptors) are large proteins which are constituent parts of numerous multicomponent repressor complexes. They function by recruiting repressors to the sites of DNA-bound transcription factors, thereby modifying chromatin condensation to restrict access for general transcription factors to a regulated gene. Both N-CoR and SMRT contain two highly conserved SANT (SWI3, ADA2, N-CoR, TFIIIB) domains which, by homology, have been suggested to confer DNA-binding properties. To date however, there have been no data published that support a DNA-binding function for N-CoR (or SMRT). To investigate if N-CoR is capable of binding to DNA, we used stably transfected S2 cells expressing FLAGtagged N-CoR We then allowed N-CoR to interact with double stranded degenerated oligonucleotides. Our results showed that oligonucleotides were retained by N-CoR, and that these contained the consensus sequence ATNNTNCTC.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-84853OAI: oai:DiVA.org:liu-84853DiVA: diva2:562546