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Fabrication of a tunable glucose biosensor based on zinc oxide/chitosan-graft-poly (vinyl alcohol) core-shell nanocomposite
(Bhaskaracharya College of Applied Sciences, University of Delhi, Delhi 10075, India)
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biosensors and Bioelectronics. Linköping University, The Institute of Technology.
2012 (English)In: Talanta, ISSN 0039-9140, Vol. 99, 283-287 p.Article in journal (Refereed) Published
Abstract [en]

A potentiometrically tuned-glucose biosensor was fabricated using core-shell nanocomposite based on zinc oxide encapsulated chitosan-graft-poly(vinyl alcohol) (ZnO/CHIT-g-PVAL). In a typical experiment, ZnO/CHIT-g-PVAL core-shell nanocomposite containing <20 nm ZnO nanoparticles was synthesized using wet-chemical method. The glucose responsive bio-electrode, i.e., glucose oxidase/ZnO/chitosan-graft-poly(vinyl alcohol) (GOD/ZnO/CHIT-g-PVAL/ITO) was obtained by immobilization of glucose oxidase (GOD) onto the electrode made of resulting ZnO core-shell nanocomposite coated on the indium-tin oxide (ITO) glass substrate. The ZnO/CHIT-g-PVAL/ITO and GOD/ZnO/CHIT-g-PVAL electrodes were characterized with Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), whereas ZnO/CHIT-g-PVAL size of core-shell nanoparticles were measured using transmission electron microscopy (TEM). The electrostatic interaction between GOD and ZnO/CHIT-g-PVAL provided the resulting tuned enzyme electrode with a high degree of enzyme immobilization and excellent lifetime stability. The response studies were carried out as a function of glucose concentration with potentiometric measurement. The GOD/ZnO/CHIT-g-PVAL/ITO bioelectrode has showed a linear potential response to the glucose concentration ranging from 2 mu M to 1.2 mM. The glucose biosensor exhibited a fast surface-controlled redox biochemistry with a detection limit of 0.2 mu M, a sensitivity of >0.04 V/mu M and a response time of three sec. ZnO/CHIT-g-PVAL core-shell nanocomposite could be a promising nanomaterials for a range of enzymic biosensors.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 99, 283-287 p.
Keyword [en]
Chitosan; Poly(vinyl alcohol); Zinc oxide; Core-shell nanocomposite; Bioelectrode; Glucose biosensor
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-85582DOI: 10.1016/j.talanta.2012.05.052ISI: 000309785300040OAI: oai:DiVA.org:liu-85582DiVA: diva2:571855
Projects
European Commission FP7 (PIIF-GA-2009-254955)
Available from: 2012-11-28 Created: 2012-11-25 Last updated: 2012-11-28Bibliographically approved

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Tiwari, Ashutosh

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