Fluorescent fibre-optic confocal imaging of lesional and non-lesional psoriatic skin compared with normal skin in vivo
2012 (English)In: Skin research and technology, ISSN 0909-752X, E-ISSN 1600-0846, Vol. 18, no 4, 397-404 p.Article in journal (Refereed) Published
Background/aims Fibre-optic confocal imaging (FOCI) allows non-invasive visualization of live skin in vivo. A contrast agent, a fluorophore, is injected in the dermis. FOCI images are optical sections from a horizontal (en face) view. The aim was to study epidermis and the cellular structure of keratinocytes of psoriatic plaques and adjacent non-lesional with healthy skin as a reference. Methods Twelve patients with stable plaque psoriasis were studied and compared with a control group of eight healthy individuals. Fluorescein sodium was used as fluorophore. A hand held fibre-optic laser scanner (Stratum (R); Optiscan Pty., Melbourne, Australia) was used. The study included morphometric analyses. Results The confocal in vivo images demonstrated characteristic features of epidermis and keratinocytes in lesional and non-lesional skin vs. healthy skin. Morphometry based on FOCI demonstrated an approximately 30% increased width of keratinocytes of psoriatic skin vs. healthy control, and the number of keratinocytes per viewing field was reduced. FOCI allowed non-invasive visualization of cell nuclei and parakeratosis of psoriatic epidermis. The horizontal width of dermal papillae of psoriatic skin was increased by approximately 50% as compared with healthy skin, and the flow of erythrocytes in the papillar vessels could be observed in real-time. Conclusion FOCI can directly visualize essential epidermal structures of plaque psoriasis in vivo, in real-time and with cellular resolution without the need of taking biopsies and thus without disturbing the natural state of the skin. FOCI is a versatile future tool for non-invasive microscopic diagnosis and therapy follow-up of psoriasis.
Place, publisher, year, edition, pages
John Wiley and Sons , 2012. Vol. 18, no 4, 397-404 p.
confocal imaging, psoriasis, in vivo, keratinocyte size, dermal papillar width, morphometry
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-85626DOI: 10.1111/j.1600-0846.2011.00585.xISI: 000309909100002OAI: oai:DiVA.org:liu-85626DiVA: diva2:572060
Funding Agencies|Welander-Finssen Foundation||2012-11-262012-11-262012-11-26