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NotI Microarrays: Novel Epigenetic Markers for Early Detection and Prognosis of High Grade Serous Ovarian Cancer
Karolinska Institute, Sweden NASU, Ukraine .
Russian Academic Science, Russia .
Russian Academic Science, Russia .
Karolinska Institute, Sweden .
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2012 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 13, no 10, 13352-13377 p.Article in journal (Refereed) Published
Abstract [en]

Chromosome 3-specific NotI microarray (NMA) containing 180 clones with 188 genes was used in the study to analyze 18 high grade serous ovarian cancer (HGSOC) samples and 7 benign ovarian tumors. We aimed to find novel methylation-dependent biomarkers for early detection and prognosis of HGSOC. Thirty five NotI markers showed frequency of methylation/deletion more or equal to 17%. To check the results of NMA hybridizations several samples for four genes (LRRC3B, THRB, ITGA9 and RBSP3 (CTDSPL)) were bisulfite sequenced and confirmed the results of NMA hybridization. A set of eight biomarkers: NKIRAS1/RPL15, THRB, RBPS3 (CTDSPL), IQSEC1, NBEAL2, ZIC4, LOC285205 and FOXP1, was identified as the most prominent set capable to detect both early and late stages of ovarian cancer. Sensitivity of this set is equal to (72 +/- 11)% and specificity (94 +/- 5)%. Early stages represented the most complicated cases for detection. To distinguish between Stages I + II and Stages III + IV of ovarian cancer the most perspective set of biomarkers would include LOC285205, CGGBP1, EPHB1 and NKIRAS1/RPL15. The sensitivity of the set is equal to (80 +/- 13)% and the specificity is (88 +/- 12)%. Using this technique we plan to validate this panel with new epithelial ovarian cancer samples and add markers from other chromosomes.

Place, publisher, year, edition, pages
MDPI , 2012. Vol. 13, no 10, 13352-13377 p.
Keyword [en]
ovarian cancer, biomarkers, NotI microarrays, epigenetics, early detection of ovarian cancer, prognosis of ovarian cancer
National Category
Social Sciences
Identifiers
URN: urn:nbn:se:liu:diva-86131DOI: 10.3390/ijms131013352ISI: 000310677800069OAI: oai:DiVA.org:liu-86131DiVA: diva2:574947
Note

Funding Agencies|Swedish Cancer Society||Swedish Institute||Swedish Research Council||Karolinska Institute||Russian Ministry of Education and Science|02.740.11.522716.552.11.7034|Russian Foundation for Basic Research|10-04-01213-a11-04-00269|

Available from: 2012-12-07 Created: 2012-12-07 Last updated: 2017-12-07

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Department of Clinical and Experimental MedicineFaculty of Health Sciences
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