liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Perioperative Betamethasone Treatment Reduces Signs of Bladder Dysfunction in a Rat Model for Neurapraxia in Female Urogenital Surgery
San Raffaele University, Italy .
San Raffaele University, Italy .
San Raffaele University, Italy .
Johns Hopkins University, MD, USA .
Show others and affiliations
2012 (English)In: European Urology, ISSN 0302-2838, E-ISSN 1873-7560, Vol. 62, no 6, 1076-1085 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

Information on autonomic neurapraxia in female urogenital surgery is scarce, and a model to study it is not available.

OBJECTIVE:

To develop a model to study the impact of autonomic neurapraxia on bladder function in female rats, as well as to assess the effects of corticosteroid therapy on the recovery of bladder function in this model.

DESIGN, SETTING, AND PARTICIPANTS:

Female Sprague-Dawley rats were subjected to bilateral pelvic nerve crush (PNC) and perioperatively treated with betamethasone or vehicle. Bladder function and morphology of bladder tissue were evaluated and compared with sham-operated rats.

OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS:

Western blot, immunohistochemistry, organ bath experiments, and cystometry.

RESULTS AND LIMITATIONS:

Sham-operated rats exhibited regular micturitions without nonvoiding contractions (NVCs). Crush of all nerve branches of the pelvic plexus or PNC resulted in overflow incontinence and/or NVCs. Betamethasone treatment improved recovery of regular micturitions (87.5% compared with 27% for vehicle; p<0.05), reduced lowest bladder pressure (8 ± 2 cm H(2)O compared with 21 ± 5 cm H(2)O for vehicle; p<0.05), and reduced the amplitude of NVCs but had no effect on NVC frequency in PNC rats. Compared with vehicle, betamethasone-treated PNC rats had less CD68 (a macrophage marker) in the pelvic plexus and bladder tissue. Isolated bladder from betamethasone-treated PNC rats exhibited better nerve-induced contractions, contained more cholinergic and sensory nerves, and expressed lower amounts of collagen III than bladder tissue from vehicle-treated rats.

CONCLUSIONS:

PNC causes autonomic neurapraxia and functional and morphologic changes of isolated bladder tissue that can be recorded as bladder dysfunction during awake cystometry in female rats. Perioperative systemic betamethasone treatment reduced macrophage contents of the pelvic plexus and bladder, partially counteracted changes in the bladder tissue, and had protective effects on micturition function.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 62, no 6, 1076-1085 p.
Keyword [en]
Model, Pelvic, Surgery, Nerve sparing, Neuropraxia, Detrusor overactivity, Corticosteroids
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-86111DOI: 10.1016/j.eururo.2012.04.037ISI: 000310783500031PubMedID: 22542670OAI: oai:DiVA.org:liu-86111DiVA: diva2:575139
Note

Funding Agencies|Swedish Medical Research Council||Gester Foundation||Urological Research Institute||

Available from: 2012-12-07 Created: 2012-12-07 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Hedlund, Petter

Search in DiVA

By author/editor
Hedlund, Petter
By organisation
Clinical PharmacologyFaculty of Health SciencesDepartment of Clinical Pharmacology
In the same journal
European Urology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 80 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf